TY - JOUR
T1 - Gene expression, and fatigue in puerto rican men during radiotherapy for prostate cancer
T2 - An exploratory study
AU - González, Velda J.
AU - Saligan, Leorey N.
AU - Fridley, Brooke L.
AU - Ortiz-Zuazaga, Humberto
AU - Aaronson, Lauren S.
N1 - Funding Information:
This article was made possible by the National Institute of Nursing Research of the National Institutes of Health under Award Number F32NR016618. Research reported in this publication was supported by the National Institute of Nursing Research Intramural Program at NIH, the ONS Foundation/ ONS Foundation Endowment Dissertation Research Grant, the Kansas University, School of Nursing, the Crighton Award, the Ruth O. McKibben Alumni Research Award, and the Sousa Award of Excellence; KU National Institute of Health grant awards P30 CA168524 and P20 GM103418R01 GM28157, U19 GM61388; and, UPR National Institute of Health grant awards 2U54MD007587 and CA096297/CA096300. The authors thank Dr. Marjorie Bott and Dr. Janet Pierce for their mentorship each step of the way on her dissertation, as well as the volunteers of this study that did not hesitate to participate.
Publisher Copyright:
© 2017, University of Puerto Rico. All rights reserved.
PY - 2017/12
Y1 - 2017/12
N2 - Objective: To examine the trajectory of fatigue experienced by 26 Puerto Rican (PR) men over the course of External Beam Radiation Therapy (EBRT) and to assess gene expression changes from baseline to midpoint of EBRT using microarray technology. Design/Research Approach- Prospective exploratory and comparative design study. Setting- RT facility located in San Juan, PR. Sample/Participants-26 PR men with non-metastatic prostate cancer. Methods: Participants completed 2 paper forms: demographics and the Spanish version of the 13-item FACT–fatigue at baseline, midpoint, and end of EBRT. Wholeblood samples were collected at baseline and at midpoint of EBRT. Descriptive data was analyzed using t-test, Wilcoxon, and Friedman test for repeated measures. Gene expression data was analyzed using the LIMMA package in R; the functional network analysis was conducted using Ingenuity Pathway analysis. Main Research Variable- Fatigue scores, gene expression. Results: Subjects were of ages 52-81 with fatigue scores that remained unchanged during EBRT (baseline=42.38, SD=9.34; midpoint=42.11, SD=8.93, endpoint=43.04, SD=8.62). Three hundred seventy-three genes (130-up regulated and 243-down regulated) were differentially expressed from baseline to mid-point of EBRT (FDR<0.01). The top distinct canonical pathways of the differentially expressed probesets (p< 0.0001) were: “Phospholipase C Signaling,” “Role of NFAT in Regulation of the Immune Response,” and “Gαq Signaling.” Conclusion: While fatigue did not worsen over the course of EBRT for this sample as a group, there was variability in fatigue across the sample. It is possible that the over expression of the SESN3 gene, known to suppress oxidative damage, may have contributed to the attenuation of fatigue in this clinical population.
AB - Objective: To examine the trajectory of fatigue experienced by 26 Puerto Rican (PR) men over the course of External Beam Radiation Therapy (EBRT) and to assess gene expression changes from baseline to midpoint of EBRT using microarray technology. Design/Research Approach- Prospective exploratory and comparative design study. Setting- RT facility located in San Juan, PR. Sample/Participants-26 PR men with non-metastatic prostate cancer. Methods: Participants completed 2 paper forms: demographics and the Spanish version of the 13-item FACT–fatigue at baseline, midpoint, and end of EBRT. Wholeblood samples were collected at baseline and at midpoint of EBRT. Descriptive data was analyzed using t-test, Wilcoxon, and Friedman test for repeated measures. Gene expression data was analyzed using the LIMMA package in R; the functional network analysis was conducted using Ingenuity Pathway analysis. Main Research Variable- Fatigue scores, gene expression. Results: Subjects were of ages 52-81 with fatigue scores that remained unchanged during EBRT (baseline=42.38, SD=9.34; midpoint=42.11, SD=8.93, endpoint=43.04, SD=8.62). Three hundred seventy-three genes (130-up regulated and 243-down regulated) were differentially expressed from baseline to mid-point of EBRT (FDR<0.01). The top distinct canonical pathways of the differentially expressed probesets (p< 0.0001) were: “Phospholipase C Signaling,” “Role of NFAT in Regulation of the Immune Response,” and “Gαq Signaling.” Conclusion: While fatigue did not worsen over the course of EBRT for this sample as a group, there was variability in fatigue across the sample. It is possible that the over expression of the SESN3 gene, known to suppress oxidative damage, may have contributed to the attenuation of fatigue in this clinical population.
KW - Cancer related fatigue
KW - Gene expression
KW - Prostate cancer
KW - Puerto Ricans
KW - Radiotherapy
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M3 - Article
C2 - 29220067
AN - SCOPUS:85037046984
VL - 36
SP - 223
EP - 231
JO - Puerto Rico Health Sciences Journal
JF - Puerto Rico Health Sciences Journal
SN - 0738-0658
IS - 4
ER -