Gene synteny and chloroquine resistance in Plasmodium chabaudi

Paul Hunt, Axel Martinelli, Richard Fawcett, Jane Carlton, Richard Carter, David Walliker

Research output: Contribution to journalArticlepeer-review

Abstract

Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume136
Issue number2
DOIs
StatePublished - Aug 2004

Keywords

  • AFLP
  • DNA
  • EDTA
  • PBS
  • PCR
  • RFLP
  • SDS
  • amplified fragment length polymorphism
  • deoxyribonucleic acid
  • ethylenediamine tetra acetic acid
  • phosphate buffered saline
  • polymerase chain reaction
  • restriction fragment length polymorphism
  • sodium dodecyl sulphate

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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