TY - JOUR
T1 - Gene synteny and chloroquine resistance in Plasmodium chabaudi
AU - Hunt, Paul
AU - Martinelli, Axel
AU - Fawcett, Richard
AU - Carlton, Jane
AU - Carter, Richard
AU - Walliker, David
N1 - Funding Information:
We wish to thank Ronnie Mooney and Les Steven for excellent technical assistance, as well as the referees of this paper for their constructive criticisms. We are grateful to the Medical Research Council and the Wellcome Trust who supported this research.
Funding Information:
Sequence data for P. falciparum chromosomes 6 and 13 were obtained from The Sanger Institute website at http://www.sanger.ac.uk/Projects/P_falciparum/ . Sequencing of P. falciparum chromosomes 6 and 13 has been accomplished as part of the Malaria Genome Project with support by The Wellcome Trust. Preliminary sequence and/or preliminary annotated sequence data from the Plasmodium yoelii genome was obtained from The Institute for Genomic Research website ( www.tigr.org ). This sequencing program is carried out in collaboration with the Naval Medical Research Center and is supported by the US Department of Defense.
PY - 2004/8
Y1 - 2004/8
N2 - Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.
AB - Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.
KW - AFLP
KW - DNA
KW - EDTA
KW - PBS
KW - PCR
KW - RFLP
KW - SDS
KW - amplified fragment length polymorphism
KW - deoxyribonucleic acid
KW - ethylenediamine tetra acetic acid
KW - phosphate buffered saline
KW - polymerase chain reaction
KW - restriction fragment length polymorphism
KW - sodium dodecyl sulphate
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U2 - 10.1016/j.molbiopara.2004.03.008
DO - 10.1016/j.molbiopara.2004.03.008
M3 - Article
C2 - 15478795
AN - SCOPUS:2942525695
SN - 0166-6851
VL - 136
SP - 157
EP - 164
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -