Generation effect of Newkome dendrimers on cellular uptake

Elizabeth A. Kaufman; Rossella Tarallo; Annarita Falanga; Stefania Galdiero; Marcus Weck

doi:10.1016/j.polymer.2017.02.040

Generation effect of Newkome dendrimers on cellular uptake

Elizabeth A. Kaufman, Rossella Tarallo, Annarita Falanga, Stefania Galdiero, Marcus Weck

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Poly(amide)-based dendrimers can be used as delivery scaffolds in conjunction with the cell-penetrating peptide gH625 derived from the glycoprotein of the Herpes Simplex virus type 1. In this contribution, we aim to isolate the optimal dendrimer generation for cellular uptake for Newkome type dendrimers conjugated with gH625. For this study, we synthesized generations zero to three of the Newkome dendrimer-gH625 bioconjugate. Fluorescent microscopy experiments showed that the second and third generations are the most efficient for cellular uptake with the second generation having the synthetic advantage. The optimal second generation can be used as an improved material for a dendrimer based delivery scaffold for peptide therapeutics.

Original language	English (US)
Pages (from-to)	67-73
Number of pages	7
Journal	Polymer
Volume	113
DOIs	https://doi.org/10.1016/j.polymer.2017.02.040
State	Published - Mar 24 2017

Keywords

Cell-uptake
Dendrimers
Polymer-bioconjugates

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.polymer.2017.02.040

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title = "Generation effect of Newkome dendrimers on cellular uptake",

abstract = "Poly(amide)-based dendrimers can be used as delivery scaffolds in conjunction with the cell-penetrating peptide gH625 derived from the glycoprotein of the Herpes Simplex virus type 1. In this contribution, we aim to isolate the optimal dendrimer generation for cellular uptake for Newkome type dendrimers conjugated with gH625. For this study, we synthesized generations zero to three of the Newkome dendrimer-gH625 bioconjugate. Fluorescent microscopy experiments showed that the second and third generations are the most efficient for cellular uptake with the second generation having the synthetic advantage. The optimal second generation can be used as an improved material for a dendrimer based delivery scaffold for peptide therapeutics.",

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T1 - Generation effect of Newkome dendrimers on cellular uptake

AU - Kaufman, Elizabeth A.

AU - Tarallo, Rossella

AU - Falanga, Annarita

AU - Galdiero, Stefania

AU - Weck, Marcus

PY - 2017/3/24

Y1 - 2017/3/24

N2 - Poly(amide)-based dendrimers can be used as delivery scaffolds in conjunction with the cell-penetrating peptide gH625 derived from the glycoprotein of the Herpes Simplex virus type 1. In this contribution, we aim to isolate the optimal dendrimer generation for cellular uptake for Newkome type dendrimers conjugated with gH625. For this study, we synthesized generations zero to three of the Newkome dendrimer-gH625 bioconjugate. Fluorescent microscopy experiments showed that the second and third generations are the most efficient for cellular uptake with the second generation having the synthetic advantage. The optimal second generation can be used as an improved material for a dendrimer based delivery scaffold for peptide therapeutics.

AB - Poly(amide)-based dendrimers can be used as delivery scaffolds in conjunction with the cell-penetrating peptide gH625 derived from the glycoprotein of the Herpes Simplex virus type 1. In this contribution, we aim to isolate the optimal dendrimer generation for cellular uptake for Newkome type dendrimers conjugated with gH625. For this study, we synthesized generations zero to three of the Newkome dendrimer-gH625 bioconjugate. Fluorescent microscopy experiments showed that the second and third generations are the most efficient for cellular uptake with the second generation having the synthetic advantage. The optimal second generation can be used as an improved material for a dendrimer based delivery scaffold for peptide therapeutics.

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KW - Dendrimers

KW - Polymer-bioconjugates

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JO - Polymer

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Generation effect of Newkome dendrimers on cellular uptake

Abstract

Keywords

ASJC Scopus subject areas

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