TY - JOUR
T1 - Genetic and genomic toolbox of the Chordate Ciona intestinalis
AU - Stolfi, Alberto
AU - Christiaen, Lionel
PY - 2012/9/1
Y1 - 2012/9/1
N2 - The experimental malleability and unique phylogenetic position of the sea squirt Ciona intestinalis as part of the sister group to the vertebrates have helped establish these marine chordates as model organisms for the study of developmental genetics and evolution. Here we summarize the tools, techniques, and resources available to the Ciona geneticist, citing examples of studies that employed such strategies in the elucidation of gene function in Ciona. Genetic screens, germline transgenesis, electroporation of plasmid DNA, and microinjection of morpholinos are all routinely employed, and in the near future we expect these to be complemented by targeted mutagenesis, homologous recombination, and RNAi. The genomic resources available will continue to support the design and interpretation of genetic experiments and allow for increasingly sophisticated approaches on a high-throughput, wholegenome scale.
AB - The experimental malleability and unique phylogenetic position of the sea squirt Ciona intestinalis as part of the sister group to the vertebrates have helped establish these marine chordates as model organisms for the study of developmental genetics and evolution. Here we summarize the tools, techniques, and resources available to the Ciona geneticist, citing examples of studies that employed such strategies in the elucidation of gene function in Ciona. Genetic screens, germline transgenesis, electroporation of plasmid DNA, and microinjection of morpholinos are all routinely employed, and in the near future we expect these to be complemented by targeted mutagenesis, homologous recombination, and RNAi. The genomic resources available will continue to support the design and interpretation of genetic experiments and allow for increasingly sophisticated approaches on a high-throughput, wholegenome scale.
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U2 - 10.1534/genetics.112.140590
DO - 10.1534/genetics.112.140590
M3 - Review article
C2 - 22964837
AN - SCOPUS:84866102966
SN - 0016-6731
VL - 192
SP - 55
EP - 66
JO - Genetics
JF - Genetics
IS - 1
ER -