Genetic variation of PLTP modulates lipoprotein profiles in hypoalphalipoproteinemia

Bradley E. Aouizerat, Mary B. Engler, Yanina Natanzon, Medha Kulkarni, James Song, Celeste Eng, Jarkko Huuskonen, Christopher Rivera, Annie Poon, Matt Bensley, Amy Sehnert, Christian Zellner, Mary Malloy, John Kane, Clive R. Pullinger

Research output: Contribution to journalArticle

Abstract

Phospholipid transfer protein (PLTP) participates in key processes in lipoprotein metabolism, including interparticle phospholipid transfer, remodeling of HDL, cholesterol and phospholipid efflux from peripheral tissues, and the production of hepatic VLDL. The impact of PLTP on reverse cholesterol transport suggests that the gene may harbor sequence anomalies that contribute to disorders of HDL metabolism. The human PLTP gene was screened for sequence anomalies by DNA melting analysis in 276 subjects with hypoalphalipoproteinemia (HA) and 364 controls. The association with plasma lipid parameters was evaluated. We discovered 18 sequence variations, including four missense mutations and a novel polymorphism (c.-34G>C). In healthy controls, the c.-34G>C minor allele was associated with higher high density lipoprotein-cholesterol (HDL-C) and was depleted in subjects with HA. Linear regression models predict that possession of the rare allele decreases plasma triglyceride (TG) and TG/HDL-C and increases HDL-C independent of TG. Decreased PLTP activity was observed in one (p.R235W) of four (p.E72G, p.S119A, p.S124Y, and p.R235W) mutations in an in vitro activity assay. These findings indicate that PLTP gene variation is an important determinant of plasma lipoproteins and affects disorders of HDL metabolism.

Original languageEnglish (US)
Pages (from-to)787-793
Number of pages7
JournalJournal of Lipid Research
Volume47
Issue number4
DOIs
StatePublished - Apr 2006

Keywords

  • Atherosclerosis
  • Cardiovascular diseases
  • Dyslipidemia
  • Genetic polymorphism
  • Phospholipid transfer protein

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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  • Cite this

    Aouizerat, B. E., Engler, M. B., Natanzon, Y., Kulkarni, M., Song, J., Eng, C., Huuskonen, J., Rivera, C., Poon, A., Bensley, M., Sehnert, A., Zellner, C., Malloy, M., Kane, J., & Pullinger, C. R. (2006). Genetic variation of PLTP modulates lipoprotein profiles in hypoalphalipoproteinemia. Journal of Lipid Research, 47(4), 787-793. https://doi.org/10.1194/jlr.M500476-JLR200