Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA.

Hannah G. Blitzblau, Andreas Hochwagen

Research output: Contribution to journalArticle

Abstract

The controlled fragmentation of chromosomes by DNA double-strand breaks (DSBs) initiates meiotic recombination, which is essential for meiotic chromosome segregation in most eukaryotes. This chapter describes a straightforward microarray-based approach to measure the genome-wide distribution of meiotic DSBs by detecting the single-stranded DNA (ssDNA) that transiently accumulates at DSB sites during recombination. The protocol outlined here has been optimized to detect meiotic DSBs in Saccharomyces cerevisiae. However, because ssDNA is a universal intermediate of homologous recombination, this method can ostensibly be adapted to discover and analyze programmed or damage-induced DSB hotspots in other organisms whose genome sequence is available.

Original languageEnglish (US)
Pages (from-to)47-63
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume745
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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