Genomic architecture of sickle cell disease in West African children

Jacklyn Quinlan, Youssef Idaghdour, Jean Philippe Goulet, Elias Gbeha, Thibault de Malliard, Vanessa Bruat, Jean Christophe Grenier, Selma Gomez, Ambaliou Sanni, Mohamed C. Rahimy, Philip Awadalla

Research output: Contribution to journalArticle

Abstract

Sickle cell disease (SCD) is a congenital blood disease, affecting predominantly children from sub-Saharan Africa, but also populations world-wide. Although the causal mutation of SCD is known, the sources of clinical variability of SCD remain poorly understood, with only a few highly heritable traits associated with SCD having been identified. Phenotypic heterogeneity in the clinical expression of SCD is problematic for follow-up (FU), management, and treatment of patients. Here we used the joint analysis of gene expression and whole genome genotyping data to identify the genetic regulatory effects contributing to gene expression variation among groups of patients exhibiting clinical variability, as well as unaffected siblings, in Benin, West Africa. We characterized and replicated patterns of whole blood gene expression variation within and between SCD patients at entry to clinic, as well as in follow-up programs. We present a global map of genes involved in the disease through analysis of whole blood sampled from the cohort. Genome-wide association mapping of gene expression revealed 390 peak genome-wide significant expression SNPs (eSNPs) and 6 significant eSNP-by-clinical status interaction effects. The strong modulation of the transcriptome implicates pathways affecting core circulating cell functions and shows how genotypic regulatory variation likely contributes to the clinical variation observed in SCD.

Original languageEnglish (US)
Article numberArticle 26
JournalFrontiers in Genetics
Volume5
Issue numberFEB
DOIs
StatePublished - 2014

Keywords

  • ESNP mapping
  • Gene-by-environment interactions
  • Genomics
  • Sickle cell disease
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Genetics(clinical)

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  • Cite this

    Quinlan, J., Idaghdour, Y., Goulet, J. P., Gbeha, E., de Malliard, T., Bruat, V., Grenier, J. C., Gomez, S., Sanni, A., Rahimy, M. C., & Awadalla, P. (2014). Genomic architecture of sickle cell disease in West African children. Frontiers in Genetics, 5(FEB), [Article 26]. https://doi.org/10.3389/fgene.2014.00026