Genomic DNA transposition induced by human PGBD5

Anton G. Henssen, Elizabeth Henaff, Eileen Jiang, Amy R. Eisenberg, Julianne R. Carson, Camila M. Villasante, Mondira Ray, Eric Still, Melissa Burns, Jorge Gandara, Cedric Feschotte, Christopher E. Mason, Alex Kentsis

Research output: Contribution to journalArticle

Abstract

Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containing inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic remodeling contributes to its biological function.

Original languageEnglish (US)
Article numbere10565
JournaleLife
Volume4
Issue numberSeptember
DOIs
StatePublished - Sep 25 2015

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Genomic DNA transposition induced by human PGBD5'. Together they form a unique fingerprint.

  • Cite this

    Henssen, A. G., Henaff, E., Jiang, E., Eisenberg, A. R., Carson, J. R., Villasante, C. M., Ray, M., Still, E., Burns, M., Gandara, J., Feschotte, C., Mason, C. E., & Kentsis, A. (2015). Genomic DNA transposition induced by human PGBD5. eLife, 4(September), [e10565]. https://doi.org/10.7554/eLife.10565