TY - JOUR
T1 - Genomic organization of the 8p11∼p12 amplicon in three breast cancer cell lines
AU - Yang, Zeng Quan
AU - Albertson, Donna
AU - Ethier, Stephen P.
N1 - Funding Information:
This work was supported by NIH grant CA70354 (S.P.E.), and DOD Breast Cancer Research Program DAMD17-03-1-0459 grant BC020649 (Z.-Q.Y). The authors would like to acknowledge Steven Kronenberg for assistance in preparing the figures.
PY - 2004/11
Y1 - 2004/11
N2 - Amplification of chromosomal regions leads to an increase of DNA copy number and expression of oncogenes in human breast cancer (HBC). Amplification of the 8p11∼p12 region occurs in 10-15% of primary, uncultured HBCs. In our panel of 11 breast cancer cells, three cell lines, SUM-44, SUM-52, and SUM-225, have overlapping amplicons in the 8p11∼p12 region. To characterize genome structure of the amplified regions, we performed fluorescence in situ hybridization using 8p11∼p12 BAC clones in the 3 cell lines. The results revealed that the 8p11∼p12 amplicon has a highly complex structure and that FGFR1 is not in the common core-amplified domain in 3 breast cancer cell lines with the amplicon. These 3 cell lines provide good models for genetic and functional studies of candidate oncogenes of the 8p11∼p12 region.
AB - Amplification of chromosomal regions leads to an increase of DNA copy number and expression of oncogenes in human breast cancer (HBC). Amplification of the 8p11∼p12 region occurs in 10-15% of primary, uncultured HBCs. In our panel of 11 breast cancer cells, three cell lines, SUM-44, SUM-52, and SUM-225, have overlapping amplicons in the 8p11∼p12 region. To characterize genome structure of the amplified regions, we performed fluorescence in situ hybridization using 8p11∼p12 BAC clones in the 3 cell lines. The results revealed that the 8p11∼p12 amplicon has a highly complex structure and that FGFR1 is not in the common core-amplified domain in 3 breast cancer cell lines with the amplicon. These 3 cell lines provide good models for genetic and functional studies of candidate oncogenes of the 8p11∼p12 region.
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U2 - 10.1016/j.cancergencyto.2004.03.013
DO - 10.1016/j.cancergencyto.2004.03.013
M3 - Article
C2 - 15527903
AN - SCOPUS:7444240784
SN - 0165-4608
VL - 155
SP - 57
EP - 62
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -