Geometry-Dependent Phosphodiester Hydrolysis Catalyzed by Binuclear Copper Complexes

Lei Zhu, Osvaldo Dos Santos, Chi Wan Koo, Marc Rybstein, Louise Pape, James W. Canary

Research output: Contribution to journalArticlepeer-review

Abstract

Two isomeric binuclear ligands PBTPA and MBTPA and their copper(II) complexes were prepared and examined for hydrolysis of a model phosphodiester substrate: bis(p-nitrophenyl) phosphate. A bell-shaped pH vs rate profile, which is in agreement with one mechanism proposed for bimetallonucleases/phosphatases, was observed for the binuclear complex of copper(II) and PBTPA. At pH 8.4, a maximum rate of 1.14 × 10-6 s-1-more than 104-fold over uncatalyzed reactions-was achieved. However, the analogous complex of MBTPA did not show significant rate enhancement. The binuclear complex of copper(II) and PBTPA also showed 10-fold acceleration over mononuclear complex of copper(II) and tris(2-pyridylmethyl)amine (TPA) catalyzed reaction. A phage φX174 DNA assay showed that the complex of copper(II) and PBTPA promoted supercoiled phage φX174 DNA relaxation under both aerobic and anaerobic conditions, in contrast to the hydrolytic inactivity of the mononuclear complex of copper(II) and TPA.

Original languageEnglish (US)
Pages (from-to)7912-7920
Number of pages9
JournalInorganic Chemistry
Volume42
Issue number24
DOIs
StatePublished - Dec 1 2003

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry

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