Global synthetic-lethality analysis and yeast functional profiling

Siew Loon Ooi, Xuewen Pan, Brian D. Peyser, Ping Ye, Pamela B. Meluh, Daniel S. Yuan, Rafael A. Irizarry, Joel S. Bader, Forrest A. Spencer, Jef D. Boeke

    Research output: Contribution to journalReview article

    Abstract

    The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

    Original languageEnglish (US)
    Pages (from-to)56-63
    Number of pages8
    JournalTrends in Genetics
    Volume22
    Issue number1
    DOIs
    StatePublished - Jan 2006

    ASJC Scopus subject areas

    • Genetics

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  • Cite this

    Ooi, S. L., Pan, X., Peyser, B. D., Ye, P., Meluh, P. B., Yuan, D. S., Irizarry, R. A., Bader, J. S., Spencer, F. A., & Boeke, J. D. (2006). Global synthetic-lethality analysis and yeast functional profiling. Trends in Genetics, 22(1), 56-63. https://doi.org/10.1016/j.tig.2005.11.003