Glycine mediated alterations in intracellular pH

Joshua S. Green; Vibhakar C. Kotak; Dan H. Sanes

doi:10.1016/S0006-8993(03)03340-7

Glycine mediated alterations in intracellular pH

Joshua S. Green, Vibhakar C. Kotak, Dan H. Sanes

Neural Science

Research output: Contribution to journal › Article › peer-review

Abstract

Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.

Original language	English (US)
Pages (from-to)	122-127
Number of pages	6
Journal	Brain Research
Volume	989
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(03)03340-7
State	Published - 2003

Keywords

Auditory
Glycine
Inhibition
pH

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(03)03340-7

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title = "Glycine mediated alterations in intracellular pH",

abstract = "Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.",

keywords = "Auditory, Glycine, Inhibition, pH",

author = "Green, {Joshua S.} and Kotak, {Vibhakar C.} and Sanes, {Dan H.}",

note = "Funding Information: Supported by NSF IBN-9630544. We thank Dr. Mitch Chesler for comments.",

year = "2003",

doi = "10.1016/S0006-8993(03)03340-7",

language = "English (US)",

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T1 - Glycine mediated alterations in intracellular pH

AU - Green, Joshua S.

AU - Kotak, Vibhakar C.

AU - Sanes, Dan H.

N1 - Funding Information: Supported by NSF IBN-9630544. We thank Dr. Mitch Chesler for comments.

PY - 2003

Y1 - 2003

N2 - Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.

AB - Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.

KW - Auditory

KW - Glycine

KW - Inhibition

KW - pH

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JO - Brain Research

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Glycine mediated alterations in intracellular pH

Abstract

Keywords

ASJC Scopus subject areas

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