Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

Anu Singh; Gaurav Sinsinbar; Meenakshi Choudhary; Veeresh Kumar; Renu Pasricha; H. N. Verma; Surinder P. Singh; Kavita Arora

doi:10.1016/j.snb.2013.05.014

Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

Anu Singh, Gaurav Sinsinbar, Meenakshi Choudhary, Veeresh Kumar, Renu Pasricha, H. N. Verma, Surinder P. Singh, Kavita Arora

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Abstract

Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).

Original language	English (US)
Pages (from-to)	675-684
Number of pages	10
Journal	Sensors and Actuators, B: Chemical
Volume	185
DOIs	https://doi.org/10.1016/j.snb.2013.05.014
State	Published - 2013

Keywords

Chitosan
DNA
Graphene oxide
Nano composite
Salmonella
Typhoid

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2013.05.014

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title = "Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid",

abstract = "Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).",

keywords = "Chitosan, DNA, Graphene oxide, Nano composite, Salmonella, Typhoid",

author = "Anu Singh and Gaurav Sinsinbar and Meenakshi Choudhary and Veeresh Kumar and Renu Pasricha and Verma, {H. N.} and Singh, {Surinder P.} and Kavita Arora",

year = "2013",

doi = "10.1016/j.snb.2013.05.014",

language = "English (US)",

volume = "185",

pages = "675--684",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

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TY - JOUR

T1 - Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

AU - Singh, Anu

AU - Sinsinbar, Gaurav

AU - Choudhary, Meenakshi

AU - Kumar, Veeresh

AU - Pasricha, Renu

AU - Verma, H. N.

AU - Singh, Surinder P.

AU - Arora, Kavita

PY - 2013

Y1 - 2013

N2 - Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).

AB - Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).

KW - Chitosan

KW - DNA

KW - Graphene oxide

KW - Nano composite

KW - Salmonella

KW - Typhoid

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VL - 185

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EP - 684

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

Abstract

Keywords

ASJC Scopus subject areas

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