TY - JOUR
T1 - Ground tissue circuitry regulates organ complexity in maize and Setaria
AU - Ortiz-Ramírez, Carlos
AU - Guillotin, Bruno
AU - Xu, Xiaosa
AU - Rahni, Ramin
AU - Zhang, Sanqiang
AU - Yan, Zhe
AU - Araujo, Poliana Coqueiro Dias
AU - Demesa-Arevalo, Edgar
AU - Lee, Laura
AU - van Eck, Joyce
AU - Gingeras, Thomas R.
AU - Jackson, David
AU - Gallagher, Kimberly L.
AU - Birnbaum, Kenneth D.
N1 - Funding Information:
K.D.B and D.J. are supported by NSF grant IOS-1934388. K.D.B, D.J., and T.R.G. were supported by NSF grant IOS 1445025. K.D.B. is funded by NIH grant R35GM136362. D.J. is funded by NSF IOS-1930101. K.L.G. is funded by NSF PGRP-23020. B.G. is funded by the Human Frontier Science Program Organization rant: LT - 000972/2018.
Publisher Copyright:
© 2021 American Association for the Advancement of Science. All rights reserved.
PY - 2021/12/3
Y1 - 2021/12/3
N2 - Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue to elaborate anatomical complexity.
AB - Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue to elaborate anatomical complexity.
KW - Flow Cytometry
KW - Genome, Plant
KW - Plant Proteins/genetics
KW - Plant Roots/cytology
KW - RNA-Seq
KW - Setaria Plant/cytology
KW - Single-Cell Analysis
KW - Transcription Factors/genetics
KW - Transcription, Genetic
KW - Zea mays/cytology
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U2 - 10.1126/science.abj2327
DO - 10.1126/science.abj2327
M3 - Article
C2 - 34855479
AN - SCOPUS:85120711805
SN - 0036-8075
VL - 374
SP - 1247
EP - 1252
JO - Science
JF - Science
IS - 6572
ER -