TY - GEN
T1 - Growing organoids and spheroids on a chip
AU - Ghassan, Amani
AU - Sahloul, Sarah
AU - Orozaliev, Ajymurat
AU - Percipalle, Piergiorgio
AU - Song, Young-Ak (Rafael)
N1 - Funding Information:
ACKNOWLEDGMENT Y.-A. Song thanks ASPRIRE Young Investigator Award (AYIA-19) for its generous funding (AYIA19-094). A. O. thanks Navajit Baban for the COMSOL simulation.
Publisher Copyright:
© 2023 IEEE.
PY - 2023
Y1 - 2023
N2 - Organoids have gained significant interests in the recent years to recapitulate organ development and disease progression in-vitro for personalized medicine. We propose a microfluidic chip design to grow organoids and spheroids of more uniform size and shape with minimal intervention. The hybrid design of the microfluidic chip allows to open and close culture wells using a reversibly bonded cover sheet. By combining the advantages of open wells similar to the conventional microplate wells and the sealed channel of microfluidic chips, cells can directly be pipetted into individual wells and continuously perfused after sealing. We demonstrated the concept of the hybrid chip by growing spheroids out of mouse embryonic fibroblasts and characterized the uniformity of their size and shape.
AB - Organoids have gained significant interests in the recent years to recapitulate organ development and disease progression in-vitro for personalized medicine. We propose a microfluidic chip design to grow organoids and spheroids of more uniform size and shape with minimal intervention. The hybrid design of the microfluidic chip allows to open and close culture wells using a reversibly bonded cover sheet. By combining the advantages of open wells similar to the conventional microplate wells and the sealed channel of microfluidic chips, cells can directly be pipetted into individual wells and continuously perfused after sealing. We demonstrated the concept of the hybrid chip by growing spheroids out of mouse embryonic fibroblasts and characterized the uniformity of their size and shape.
KW - lab-on-a-chip
KW - organoids
KW - spheroids
UR - http://www.scopus.com/inward/record.url?scp=85168241211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85168241211&partnerID=8YFLogxK
U2 - 10.1109/NEMS57332.2023.10190866
DO - 10.1109/NEMS57332.2023.10190866
M3 - Conference contribution
AN - SCOPUS:85168241211
T3 - 18th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2023
SP - 153
EP - 156
BT - 18th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2023
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 18th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2023
Y2 - 14 May 2023 through 17 May 2023
ER -