Heart-liver on a chip integrated with a microelectrode array to monitor extracellular field potentials of cardiomyocytes

Dongxiao Zhang, Yoshikazu Hirai, Ken Ichiro Kamei, Osamu Tabata, Toshiyuki Tsuchiya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Body-on-a-chip (BOC) replicate human physiological conditions by co-culturing human cell lines interconnected via a medium re-circulation. In this paper, we design and fabricate an integrated Heart-Liver on a chip with a microelectrode array (MEA) since a major challenge in BOC remains to monitor electrophysiological activities of cardiomyocytes in real-time upon the treatment of drug candidates. The MEA encapsulated by parylene-C films was embedded in polydimethylsiloxane, that had enabled to seamlessly integrate into BOC having a pneumatic pump. The impedance magnitude of MEA measured under the condition of medium re-circulation revealed a potential for recording field potential of cardiac electrical activities.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages941-942
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • Body on a Chip
  • Microelectrode array
  • Parylene-C
  • Polydimethylsiloxane
  • Soft lithography

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • General Chemistry
  • Control and Systems Engineering

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