TY - JOUR
T1 - Helical complexes of polyriboinosinic acid with copolymers of polyribocytidylic acid containing inosine, adenosine and uridine residues
AU - Wang, Amy C.
AU - Kallenbach, Neville R.
N1 - Funding Information:
This research was supported by National Science Foundation grant no. GB-11782. The authors wish to acknowledge helpful correspondence with Dr H. Lozeron concerning the mixed gradient system, and the capable technical assistance of Mrs S. Stefanovic in the equilibrium centrifugation experiments.
PY - 1971/12/28
Y1 - 1971/12/28
N2 - The consequences of incorporating non-complementary residues into the poly (I) · poly (C) helix have been investigated. Complexes of poly (I) and copolymers of C with different mole-ratios of I, A and U residues have been prepared and denatured in a variety of solvents. The results of both denaturation and analysis of the stoichiometry of the reactions suggest that in poly (I)· poly (C, Ix) complexes, the I residues are excluded from the helix matrix, whereas in the poly (I) · poly (C, Ux) and poly (I) · poly (C, Ax) systems the minor component bases are retained. Preliminaries to a quantitative analysis of the transition data are presented, permitting rough estimates of the difference in stability between poly (I) · poly (C) and poly (I) · poly (U) or poly (I) · poly (A) pairs in these complexes-the results being 1.7 kcal./mole and 1.3 kcal./mole, respectively. The differences in behavior of poly (I) · poly (C, I) complexes are found to be most evident in the presence of 8 m-urea.
AB - The consequences of incorporating non-complementary residues into the poly (I) · poly (C) helix have been investigated. Complexes of poly (I) and copolymers of C with different mole-ratios of I, A and U residues have been prepared and denatured in a variety of solvents. The results of both denaturation and analysis of the stoichiometry of the reactions suggest that in poly (I)· poly (C, Ix) complexes, the I residues are excluded from the helix matrix, whereas in the poly (I) · poly (C, Ux) and poly (I) · poly (C, Ax) systems the minor component bases are retained. Preliminaries to a quantitative analysis of the transition data are presented, permitting rough estimates of the difference in stability between poly (I) · poly (C) and poly (I) · poly (U) or poly (I) · poly (A) pairs in these complexes-the results being 1.7 kcal./mole and 1.3 kcal./mole, respectively. The differences in behavior of poly (I) · poly (C, I) complexes are found to be most evident in the presence of 8 m-urea.
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U2 - 10.1016/0022-2836(71)90158-6
DO - 10.1016/0022-2836(71)90158-6
M3 - Article
C2 - 5136583
AN - SCOPUS:0015243882
SN - 0022-2836
VL - 62
SP - 591
EP - 607
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 3
ER -