TY - JOUR
T1 - Herringbone Microfluidic Probe for Multiplexed Affinity-Capture of Prostate Circulating Tumor Cells
AU - Glia, Ayoub
AU - Deliorman, Muhammedin
AU - Sukumar, Pavithra
AU - Janahi, Farhad K.
AU - Samara, Bisan
AU - Brimmo, Ayoola T.
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2021 The Authors. Advanced Materials Technologies published by Wiley-VCH GmbH
PY - 2021/6
Y1 - 2021/6
N2 - In this work, for first time, circulating tumor cells (CTCs) are captured on an open biofunctionalized substrate with multiplexing capability. This is achieved by developing a new microfluidic probe (MFP) integrated with radially staggered herringbone (HB) elements for microvortex generation. The new tool, named as herringbone microfluidic probe (HB-MFP), is a channel-less microfluidic system with physically separated bottom capture substrate and top fluidics delivery system. The concept allows for functionalizing the capture substrate with multiple biorecognition ligands (in this work, stripes of different capture antibodies) and scanning the fluidics delivery system across the substrate in a 2D printing-like movement. Using the HB-MFP, CTCs are efficiently captured from prostate cancer blood samples through their specific EpCAM, PSMA, and PSA antigens in a single run, with counts ranging from as low as 6 CTCs mL-1 (localized cancer patients) to as high as 280 CTCs mL-1 (metastatic cancer patients). In the process, CTC clusters with sizes of as high as 40–50 cells are also successfully captured. The results indicate that multiplex profiles of CTCs could reveal certain cellular phenotypes based on PSMA and PSA expression levels. The developed HB-MFP is simple and robust to use, allows for high throughput sample processing, and provides seamless access to captured CTCs for further downstream characterization.
AB - In this work, for first time, circulating tumor cells (CTCs) are captured on an open biofunctionalized substrate with multiplexing capability. This is achieved by developing a new microfluidic probe (MFP) integrated with radially staggered herringbone (HB) elements for microvortex generation. The new tool, named as herringbone microfluidic probe (HB-MFP), is a channel-less microfluidic system with physically separated bottom capture substrate and top fluidics delivery system. The concept allows for functionalizing the capture substrate with multiple biorecognition ligands (in this work, stripes of different capture antibodies) and scanning the fluidics delivery system across the substrate in a 2D printing-like movement. Using the HB-MFP, CTCs are efficiently captured from prostate cancer blood samples through their specific EpCAM, PSMA, and PSA antigens in a single run, with counts ranging from as low as 6 CTCs mL-1 (localized cancer patients) to as high as 280 CTCs mL-1 (metastatic cancer patients). In the process, CTC clusters with sizes of as high as 40–50 cells are also successfully captured. The results indicate that multiplex profiles of CTCs could reveal certain cellular phenotypes based on PSMA and PSA expression levels. The developed HB-MFP is simple and robust to use, allows for high throughput sample processing, and provides seamless access to captured CTCs for further downstream characterization.
KW - antigen–antibody capture
KW - circulating tumor cells
KW - herringbone chaotic mixing
KW - liquid biopsy
KW - microfluidics
KW - scanning probes
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U2 - 10.1002/admt.202100053
DO - 10.1002/admt.202100053
M3 - Article
AN - SCOPUS:85104385926
SN - 2365-709X
VL - 6
JO - Advanced Materials Technologies
JF - Advanced Materials Technologies
IS - 6
M1 - 2100053
ER -