TY - GEN
T1 - Hexagonal Microfluidic Mixing Probe for Flow Concentration Gradients on Suspended Cells
AU - Ali, Dima S.
AU - Waheed, Waqas
AU - Glia, Ayoub
AU - Sukumar, Pavithra
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2024 IEEE.
PY - 2024
Y1 - 2024
N2 - In this study, we introduce a novel hexagonal microfluidic mixing probe (MMP) that combines the concepts of microfluidic probe (MFP) and Christmas-tree concentration gradient generators.The advanced MMP generates six concentric hydrodynamic flow confinements (HFC) with five distinct reagent concentrations.The system integrates an Open micro-electro-fluidic (OMEF) biochip of interdigitated electrodes (IDE) positioned below the HFC footprint.Additionally, the electrically activated bottom substrate employs dielectrophoretic (DEP) force for frequency-based selective capturing of suspended cells.The MMP-generated HFCs are positioned atop the DEP-captured suspended cells, exposing them simultaneously to six distinct reagent concentrations.This system enables the execution of multiplex spatiotemporally controlled experiments to assess the effects of various reagent concentrations and their combinations.This versatile tool promises to revolutionize drug dosage precision, potentially leading to a more efficient and cost-effective preclinical testing protocol.
AB - In this study, we introduce a novel hexagonal microfluidic mixing probe (MMP) that combines the concepts of microfluidic probe (MFP) and Christmas-tree concentration gradient generators.The advanced MMP generates six concentric hydrodynamic flow confinements (HFC) with five distinct reagent concentrations.The system integrates an Open micro-electro-fluidic (OMEF) biochip of interdigitated electrodes (IDE) positioned below the HFC footprint.Additionally, the electrically activated bottom substrate employs dielectrophoretic (DEP) force for frequency-based selective capturing of suspended cells.The MMP-generated HFCs are positioned atop the DEP-captured suspended cells, exposing them simultaneously to six distinct reagent concentrations.This system enables the execution of multiplex spatiotemporally controlled experiments to assess the effects of various reagent concentrations and their combinations.This versatile tool promises to revolutionize drug dosage precision, potentially leading to a more efficient and cost-effective preclinical testing protocol.
KW - 3D printing
KW - Concentration gradient generator
KW - Dielectrophoresis (DEP)
KW - Interdigitated electrodes (IDE)
KW - Microfluidic probe
UR - http://www.scopus.com/inward/record.url?scp=85202341927&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85202341927&partnerID=8YFLogxK
U2 - 10.1109/MARSS61851.2024.10612715
DO - 10.1109/MARSS61851.2024.10612715
M3 - Conference contribution
AN - SCOPUS:85202341927
T3 - Proceedings of MARSS 2024 - 7th International Conference on Manipulation, Automation, and Robotics at Small Scales
BT - Proceedings of MARSS 2024 - 7th International Conference on Manipulation, Automation, and Robotics at Small Scales
A2 - Haliyo, Sinan
A2 - Boudaoud, Mokrane
A2 - Mastrangeli, Massimo
A2 - Lambert, Pierre
A2 - Fatikow, Sergej
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 7th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2024
Y2 - 1 July 2024 through 5 July 2024
ER -