Hierarchical herringbone microwells for high-throughput single cell entrapment

Ayoub Glia, Pavithra Sukumar, Muhammedin Deliorman, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here, we developed a multichannel microfluidic chip that consists of hierarchical herringbone (HH)-microwells, staggered on both sides of its channels, for efficient entrapment of cancer cells in a single cell per microwell format. In addition to a single common cell loading inlet, the chip also has three separate drug loading inlets on its other side for drug testing applications. We show, by simulation and experimentally, that the micromixing induced by HH-microwells (totaling 5400 per channel) entraps cells within the bare microwells at ~80-85% efficiency. Then the chip is demonstrated in evaluating the susceptibility of isolated cancer cells to cisplatin chemotherapy.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1147-1148
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • 3D Printing
  • Drug Testing
  • Hierarchical Herringbone Microwells
  • Single Cell Isolation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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