TY - GEN
T1 - Hierarchical herringbone microwells for high-throughput single cell entrapment
AU - Glia, Ayoub
AU - Sukumar, Pavithra
AU - Deliorman, Muhammedin
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Here, we developed a multichannel microfluidic chip that consists of hierarchical herringbone (HH)-microwells, staggered on both sides of its channels, for efficient entrapment of cancer cells in a single cell per microwell format. In addition to a single common cell loading inlet, the chip also has three separate drug loading inlets on its other side for drug testing applications. We show, by simulation and experimentally, that the micromixing induced by HH-microwells (totaling 5400 per channel) entraps cells within the bare microwells at ~80-85% efficiency. Then the chip is demonstrated in evaluating the susceptibility of isolated cancer cells to cisplatin chemotherapy.
AB - Here, we developed a multichannel microfluidic chip that consists of hierarchical herringbone (HH)-microwells, staggered on both sides of its channels, for efficient entrapment of cancer cells in a single cell per microwell format. In addition to a single common cell loading inlet, the chip also has three separate drug loading inlets on its other side for drug testing applications. We show, by simulation and experimentally, that the micromixing induced by HH-microwells (totaling 5400 per channel) entraps cells within the bare microwells at ~80-85% efficiency. Then the chip is demonstrated in evaluating the susceptibility of isolated cancer cells to cisplatin chemotherapy.
KW - 3D Printing
KW - Drug Testing
KW - Hierarchical Herringbone Microwells
KW - Single Cell Isolation
UR - http://www.scopus.com/inward/record.url?scp=85098266819&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098266819&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098266819
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1147
EP - 1148
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -