@article{79356652336844579f993fcad670be80,
title = "High-definition spatial transcriptomics for in situ tissue profiling",
abstract = "Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.",
author = "Sanja Vickovic and G{\"o}kcen Eraslan and Fredrik Salm{\'e}n and Johanna Klughammer and Linnea Stenbeck and Denis Schapiro and Tarmo {\"A}ij{\"o} and Richard Bonneau and Ludvig Bergenstr{\aa}hle and Navarro, {Jos{\'e} Fernand{\'e}z} and Joshua Gould and Griffin, {Gabriel K.} and {\AA}ke Borg and Mostafa Ronaghi and Jonas Fris{\'e}n and Joakim Lundeberg and Aviv Regev and St{\aa}hl, {Patrik L.}",
note = "Funding Information: We thank the NGI Stockholm, SciLifeLab, Flatiron Institute and Simons Foundation for providing infrastructure support. We thank L. Gaffney, A. Hupalowska and J. Rood for help with manuscript preparation. Work was supported by the Knut and Alice Wallenberg Foundation, Swedish Foundation for Strategic Research, Swedish Cancer Society and the Swedish Research Council (to S.V., J.L. and P.L.S.), the EMBO long-term fellowship (no. ALTF 738-2017) to J.K., Early Postdoc Mobility fellowship (no. P2ZHP3_181475) to D.S., the NIH HuBMAP HIVE project and the Klarman Cell Observatory and HHMI (to A.R.). S.V is supported as a Wallenberg Fellow at the Broad Institute of MIT and Harvard. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2019",
month = oct,
day = "1",
doi = "10.1038/s41592-019-0548-y",
language = "English (US)",
volume = "16",
pages = "987--990",
journal = "Nature methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "10",
}