High-definition spatial transcriptomics for in situ tissue profiling

Sanja Vickovic; Gökcen Eraslan; Fredrik Salmén; Johanna Klughammer; Linnea Stenbeck; Denis Schapiro; Tarmo Äijö; Richard Bonneau; Ludvig Bergenstråhle; José Fernandéz Navarro; Joshua Gould; Gabriel K. Griffin; Åke Borg; Mostafa Ronaghi; Jonas Frisén; Joakim Lundeberg; Aviv Regev; Patrik L. Ståhl

doi:10.1038/s41592-019-0548-y

High-definition spatial transcriptomics for in situ tissue profiling

Sanja Vickovic, Gökcen Eraslan, Fredrik Salmén, Johanna Klughammer, Linnea Stenbeck, Denis Schapiro, Tarmo Äijö, Richard Bonneau, Ludvig Bergenstråhle, José Fernandéz Navarro, Joshua Gould, Gabriel K. Griffin, Åke Borg, Mostafa Ronaghi, Jonas Frisén, Joakim Lundeberg, Aviv Regev, Patrik L. Ståhl

Research output: Contribution to journal › Article

Abstract

Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.

Original language	English (US)
Pages (from-to)	987-990
Number of pages	4
Journal	Nature methods
Volume	16
Issue number	10
DOIs	https://doi.org/10.1038/s41592-019-0548-y
State	Published - Oct 1 2019

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Vickovic, S., Eraslan, G., Salmén, F., Klughammer, J., Stenbeck, L., Schapiro, D., Äijö, T., Bonneau, R., Bergenstråhle, L., Navarro, J. F., Gould, J., Griffin, G. K., Borg, Å., Ronaghi, M., Frisén, J., Lundeberg, J., Regev, A., & Ståhl, P. L. (2019). High-definition spatial transcriptomics for in situ tissue profiling. Nature methods, 16(10), 987-990. https://doi.org/10.1038/s41592-019-0548-y

High-definition spatial transcriptomics for in situ tissue profiling. / Vickovic, Sanja; Eraslan, Gökcen; Salmén, Fredrik; Klughammer, Johanna; Stenbeck, Linnea; Schapiro, Denis; Äijö, Tarmo; Bonneau, Richard; Bergenstråhle, Ludvig; Navarro, José Fernandéz; Gould, Joshua; Griffin, Gabriel K.; Borg, Åke; Ronaghi, Mostafa; Frisén, Jonas; Lundeberg, Joakim; Regev, Aviv; Ståhl, Patrik L.

In: Nature methods, Vol. 16, No. 10, 01.10.2019, p. 987-990.

Research output: Contribution to journal › Article

Vickovic, S, Eraslan, G, Salmén, F, Klughammer, J, Stenbeck, L, Schapiro, D, Äijö, T, Bonneau, R, Bergenstråhle, L, Navarro, JF, Gould, J, Griffin, GK, Borg, Å, Ronaghi, M, Frisén, J, Lundeberg, J, Regev, A & Ståhl, PL 2019, 'High-definition spatial transcriptomics for in situ tissue profiling', Nature methods, vol. 16, no. 10, pp. 987-990. https://doi.org/10.1038/s41592-019-0548-y

Vickovic, Sanja ; Eraslan, Gökcen ; Salmén, Fredrik ; Klughammer, Johanna ; Stenbeck, Linnea ; Schapiro, Denis ; Äijö, Tarmo ; Bonneau, Richard ; Bergenstråhle, Ludvig ; Navarro, José Fernandéz ; Gould, Joshua ; Griffin, Gabriel K. ; Borg, Åke ; Ronaghi, Mostafa ; Frisén, Jonas ; Lundeberg, Joakim ; Regev, Aviv ; Ståhl, Patrik L. / High-definition spatial transcriptomics for in situ tissue profiling. In: Nature methods. 2019 ; Vol. 16, No. 10. pp. 987-990.

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