High-frequency deletion between homologous sequences during retrotransposition of Ty elements in Saccharomyces cerevisiae.

H. Xu, J. D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    By following the fates of genetically marked Ty elements, we observed a very high frequency (80-90%) of deletion between directly repeated marker sequences during transposition. From blot hybridization analyses of Ty RNA and DNA species found in the Ty virus-like particles, we determined that the deletion events occurred during or immediately after reverse transcription of Ty RNA but before integration of Ty DNA. The results suggest that the Ty reverse-transcription machinery can recognize homologous sequences in the template. This capacity may be utilized in the replication and recombination processes of retrotransposons and retroviruses.

    Original languageEnglish (US)
    Pages (from-to)8553-8557
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume84
    Issue number23
    DOIs
    StatePublished - Dec 1987

    ASJC Scopus subject areas

    • General

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