TY - JOUR
T1 - High Quality Genome-Wide Genotyping from Archived Dried Blood Spots without DNA Amplification
AU - St. Julien, Krystal R.
AU - Jelliffe-Pawlowski, Laura L.
AU - Shaw, Gary M.
AU - Stevenson, David K.
AU - O'Brodovich, Hugh M.
AU - Krasnow, Mark A.
PY - 2013/5/30
Y1 - 2013/5/30
N2 - Spots of blood are routinely collected from newborn babies onto filter paper called Guthrie cards and used to screen for metabolic and genetic disorders. The archived dried blood spots are an important and precious resource for genomic research. Whole genome amplification of dried blood spot DNA has been used to provide DNA for genome-wide SNP genotyping. Here we describe a 96 well format procedure to extract DNA from a portion of a dried blood spot that provides sufficient unamplified genomic DNA for genome-wide single nucleotide polymorphism (SNP) genotyping. We show that SNP genotyping of the unamplified DNA is more robust than genotyping amplified dried blood spot DNA, is comparable in cost, and can be done with thousands of samples. This procedure can be used for genome-wide association studies and other large-scale genomic analyses that require robust, high-accuracy genotyping of dried blood spot DNA.
AB - Spots of blood are routinely collected from newborn babies onto filter paper called Guthrie cards and used to screen for metabolic and genetic disorders. The archived dried blood spots are an important and precious resource for genomic research. Whole genome amplification of dried blood spot DNA has been used to provide DNA for genome-wide SNP genotyping. Here we describe a 96 well format procedure to extract DNA from a portion of a dried blood spot that provides sufficient unamplified genomic DNA for genome-wide single nucleotide polymorphism (SNP) genotyping. We show that SNP genotyping of the unamplified DNA is more robust than genotyping amplified dried blood spot DNA, is comparable in cost, and can be done with thousands of samples. This procedure can be used for genome-wide association studies and other large-scale genomic analyses that require robust, high-accuracy genotyping of dried blood spot DNA.
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U2 - 10.1371/journal.pone.0064710
DO - 10.1371/journal.pone.0064710
M3 - Article
C2 - 23737996
AN - SCOPUS:84878495450
SN - 1932-6203
VL - 8
JO - PloS one
JF - PloS one
IS - 5
M1 - e64710
ER -