High-resolution analysis of paraffin-embedded and formalin-fixed prostate tumors using comparative genomic hybridization to genomic microarrays

Pamela L. Paris, Donna G. Albertson, Janneke C. Alers, Armann Andaya, Peter Carroll, Jane Fridlyand, Ajay N. Jain, Sherwin Kamkar, David Kowbel, Pieter Jaap Krijtenburg, Daniel Pinkel, Fritz H. Schröder, Kees J. Vissers, Vivienne J.E. Watson, Mark F. Wildhagen, Colin Collins, Herman Van Dekkent

Research output: Contribution to journalArticle

Abstract

We have used prostate cancer, the most commonly diagnosed noncutaneous neoplasm among men, to investigate the feasibility of performing genomic array analyses of archival tissue. Prostate-specific antigen and a biopsy Gleason grade have not proven to be accurate in predicting clinical outcome, yet they remain the only accepted biomarkers for prostate cancer. It is likely that distinct spectra of genomic alterations underlie these phenotypic differences, and that once identified, may be used to differentiate between indolent and aggressive tumors. Array comparative genomic hybridization allows quantitative detection and mapping of copy number aberrations in tumors and subsequent associations to be made with clinical outcome. Archived tissues are needed to have patients with sufficient clinical follow-up. In this report, 20 formalin-fixed and paraffin-embedded prostate cancer samples originating from 1986 to 1996 were studied. We present a straightforward protocol and demonstrate the utility of archived tissue for array comparative genomic hybridization with a 2400 element BAC array that provides high-resolution detection of both deletions and amplifications.

Original languageEnglish (US)
Pages (from-to)763-770
Number of pages8
JournalAmerican Journal of Pathology
Volume162
Issue number3
DOIs
StatePublished - Mar 1 2003

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Paris, P. L., Albertson, D. G., Alers, J. C., Andaya, A., Carroll, P., Fridlyand, J., Jain, A. N., Kamkar, S., Kowbel, D., Krijtenburg, P. J., Pinkel, D., Schröder, F. H., Vissers, K. J., Watson, V. J. E., Wildhagen, M. F., Collins, C., & Van Dekkent, H. (2003). High-resolution analysis of paraffin-embedded and formalin-fixed prostate tumors using comparative genomic hybridization to genomic microarrays. American Journal of Pathology, 162(3), 763-770. https://doi.org/10.1016/S0002-9440(10)63873-4