High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping

Weiwen Cai; Junping Jing; Benjamin Irvin; Lynne Ohler; Elise Rose; Hiroaki Shizuya; Ung Jim Kim; Melvin Simon; Thomas Anantharaman; Bhubaneswar Mishra; David C. Schwartz

doi:10.1073/pnas.95.7.3390

High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping

Weiwen Cai, Junping Jing, Benjamin Irvin, Lynne Ohler, Elise Rose, Hiroaki Shizuya, Ung Jim Kim, Melvin Simon, Thomas Anantharaman, Bhubaneswar Mishra, David C. Schwartz

Computer Science

Research output: Contribution to journal › Article › peer-review

Abstract

Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being 'redundant' in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.

Original language	English (US)
Pages (from-to)	3390-3395
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	95
Issue number	7
DOIs	https://doi.org/10.1073/pnas.95.7.3390
State	Published - Mar 31 1998

Keywords

DNA sequencing
Fluorescence microscopy
Genome mapping
Restriction endonuclease

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.95.7.3390

Cite this

Cai, W., Jing, J., Irvin, B., Ohler, L., Rose, E., Shizuya, H., Kim, U. J., Simon, M., Anantharaman, T., Mishra, B., & Schwartz, D. C. (1998). High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping. Proceedings of the National Academy of Sciences of the United States of America, 95(7), 3390-3395. https://doi.org/10.1073/pnas.95.7.3390

Cai, W, Jing, J, Irvin, B, Ohler, L, Rose, E, Shizuya, H, Kim, UJ, Simon, M, Anantharaman, T, Mishra, B & Schwartz, DC 1998, 'High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping', Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 7, pp. 3390-3395. https://doi.org/10.1073/pnas.95.7.3390

@article{bf85f6efcc9d4970b606238bb2f60ef6,

title = "High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping",

abstract = "Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being 'redundant' in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.",

keywords = "DNA sequencing, Fluorescence microscopy, Genome mapping, Restriction endonuclease",

author = "Weiwen Cai and Junping Jing and Benjamin Irvin and Lynne Ohler and Elise Rose and Hiroaki Shizuya and Kim, {Ung Jim} and Melvin Simon and Thomas Anantharaman and Bhubaneswar Mishra and Schwartz, {David C.}",

year = "1998",

month = mar,

day = "31",

doi = "10.1073/pnas.95.7.3390",

language = "English (US)",

volume = "95",

pages = "3390--3395",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "7",

}

TY - JOUR

T1 - High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping

AU - Cai, Weiwen

AU - Jing, Junping

AU - Irvin, Benjamin

AU - Ohler, Lynne

AU - Rose, Elise

AU - Shizuya, Hiroaki

AU - Kim, Ung Jim

AU - Simon, Melvin

AU - Anantharaman, Thomas

AU - Mishra, Bhubaneswar

AU - Schwartz, David C.

PY - 1998/3/31

Y1 - 1998/3/31

N2 - Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being 'redundant' in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.

AB - Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being 'redundant' in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.

KW - DNA sequencing

KW - Fluorescence microscopy

KW - Genome mapping

KW - Restriction endonuclease

UR - http://www.scopus.com/inward/record.url?scp=13144259632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13144259632&partnerID=8YFLogxK

U2 - 10.1073/pnas.95.7.3390

DO - 10.1073/pnas.95.7.3390

M3 - Article

C2 - 9520376

AN - SCOPUS:13144259632

SN - 0027-8424

VL - 95

SP - 3390

EP - 3395

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 7

ER -

High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this