High-throughput mechanical phenotyping of C. elegans diabetes models using elastomeric micropillar arrays

Samuel Sofela, Sarah Sahloul, Christopher Stubbs, Ajymurat Orozaliev, Yong Ak Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using an elastomeric micropillar array on chip, we studied the thrashing force degradation of Caenorhabditis elegans, both wild type and diabetes models, in hyperglycemic conditions. First, we established a quantitative relationship between the magnitude of thrashing force loss and glucose concentration with and without drug treatment. A change in sarcomere morphology of the actin filaments in the body wall muscles supported our finding. Finally, we demonstrated the multiplexing capability of the device by incorporating eight parallel channels for measuring eight worms simultaneously, thereby significantly increasing the throughput.

Original languageEnglish (US)
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages384-385
Number of pages2
ISBN (Electronic)9781733419000
StatePublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period10/27/1910/31/19

Keywords

  • C. elegans
  • High-throughput drug screening
  • Micropillars
  • Thrashing force

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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