High-throughput proteomic sample preconcentration in pdms microfluidic chip using surface-patterned ion-selective membrane

Jeong Hoon Lee, Yong Ak Song, Sung Jae Kim, Jongyoon Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a novel method of fabricating a high-throughput protein preconcentrator in PDMS microfluidic chip format. Using microstamping or microflow patterning of ion-selective resin, we can build a submicron thick ion-selective membrane on the glass substrate. By simply plasma bonding a PDMS device on top of the thin membrane, we can rapidly prototype a PDMS preconcentrator without complicated microfabrication processes. The PDMS preconcentrator shows a highly efficient preconcentration with a factor as high as ~104 in 5 min. Our fabrication method even allows building a massively parallel array of preconcentrators to increase the concentrated sample volume, which facilitates the integration of preconcentrator into various detectors.

Original languageEnglish (US)
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1198-1200
Number of pages3
ISBN (Electronic)9780979806407
StatePublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: Oct 7 2007Oct 11 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period10/7/0710/11/07

Keywords

  • Ion-selective membrane
  • Microcontact printing
  • Preconcentration
  • Protein

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Lee, J. H., Song, Y. A., Kim, S. J., & Han, J. (2007). High-throughput proteomic sample preconcentration in pdms microfluidic chip using surface-patterned ion-selective membrane. In J-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 1198-1200). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.