High-throughput sample preparation for mass spectrometry by continuousflow pi-based fractionation of peptides and proteins

Yong Ak Song, Jongyoon Han

Research output: Contribution to conferencePaper

Abstract

Mass spectrometry (MS) is an indispensable technique for the analysis of a wide range of molecules in proteomics. To fully exploit its analytical capability, highthroughput and efficient sample preparation is of critical importance. We have developed a continuous-flow isoelectric point (pI)-based sorting device in PDMS chip format. This device allows continuous sorting of the biomolecules according to their pI values based on the free-flow zone electrophoresis, and facilitates straightforward integration to MS.

Original languageEnglish (US)
Pages1323-1325
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Free-flow zone electrophoresis
  • IEF
  • Proteomics
  • Separation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Song, Y. A., & Han, J. (2008). High-throughput sample preparation for mass spectrometry by continuousflow pi-based fractionation of peptides and proteins. 1323-1325. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.