High-throughput sample preparation for mass spectrometry by continuousflow pi-based fractionation of peptides and proteins

Yong Ak Song; Jongyoon Han

High-throughput sample preparation for mass spectrometry by continuousflow pi-based fractionation of peptides and proteins

Mechanical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Mass spectrometry (MS) is an indispensable technique for the analysis of a wide range of molecules in proteomics. To fully exploit its analytical capability, highthroughput and efficient sample preparation is of critical importance. We have developed a continuous-flow isoelectric point (pI)-based sorting device in PDMS chip format. This device allows continuous sorting of the biomolecules according to their pI values based on the free-flow zone electrophoresis, and facilitates straightforward integration to MS.

Original language	English (US)
Pages	1323-1325
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Free-flow zone electrophoresis
IEF
Proteomics
Separation

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

High-throughput sample preparation for mass spectrometry by continuousflow pi-based fractionation of peptides and proteins. / Song, Yong Ak; Han, Jongyoon.
2008. 1323-1325 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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AU - Song, Yong Ak

AU - Han, Jongyoon

PY - 2008

Y1 - 2008

N2 - Mass spectrometry (MS) is an indispensable technique for the analysis of a wide range of molecules in proteomics. To fully exploit its analytical capability, highthroughput and efficient sample preparation is of critical importance. We have developed a continuous-flow isoelectric point (pI)-based sorting device in PDMS chip format. This device allows continuous sorting of the biomolecules according to their pI values based on the free-flow zone electrophoresis, and facilitates straightforward integration to MS.

AB - Mass spectrometry (MS) is an indispensable technique for the analysis of a wide range of molecules in proteomics. To fully exploit its analytical capability, highthroughput and efficient sample preparation is of critical importance. We have developed a continuous-flow isoelectric point (pI)-based sorting device in PDMS chip format. This device allows continuous sorting of the biomolecules according to their pI values based on the free-flow zone electrophoresis, and facilitates straightforward integration to MS.

KW - Free-flow zone electrophoresis

KW - IEF

KW - Proteomics

KW - Separation

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

High-throughput sample preparation for mass spectrometry by continuousflow pi-based fractionation of peptides and proteins

Abstract

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Keywords

ASJC Scopus subject areas

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