TY - JOUR
T1 - High-throughput sorting of eggs for synchronization of
T2 - C. elegans in a microfluidic spiral chip
AU - Sofela, Samuel
AU - Sahloul, Sarah
AU - Rafeie, Mehdi
AU - Kwon, Taehong
AU - Han, Jongyoon
AU - Warkiani, Majid Ebrahimi
AU - Song, Yong Ak
N1 - Funding Information:
We would like to acknowledge Prof. Kristin Gunsalus and her team (Fathima Shaffra Mohammed, Rawan Kalloush and Dr. Hala Fahs) of the Center for Genomics and Systems Biology, NYU Abu Dhabi for their support in the culture of the C. elegans used in this work. We gratefully acknowledge the grant support for this work from the Al Jalila Foundation (AJF201633), Dubai UAE. M. E. W. would like to acknowledge the support of the Australian Research Council through a Discovery Project Grant (DP170103704). This work was supported by Singapore-MIT Alliance for Research and Technology (SMART) Centre BioSyM IRG, as well as SMART Innovation Centre grants (ING137075-BIO and ING1510101-BIO). T. K. was partially supported by a Samsung Scholarship.
Publisher Copyright:
© 2018 The Royal Society of Chemistry.
PY - 2018/2/21
Y1 - 2018/2/21
N2 - In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min-1. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min-1), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.
AB - In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min-1. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min-1), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.
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U2 - 10.1039/c7lc00998d
DO - 10.1039/c7lc00998d
M3 - Article
C2 - 29372209
AN - SCOPUS:85042132109
SN - 1473-0197
VL - 18
SP - 679
EP - 687
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
IS - 4
ER -