Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip

Takashi Miyazaki; Jiandong Yang; Satoshi Imamura; Yoshikazu Hirai; Ken Ichiro Kamei; Toshiyuki Tsuchiya; Osamu Tabata

doi:10.1109/MEMS51782.2021.9375164

Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip

Takashi Miyazaki, Jiandong Yang, Satoshi Imamura, Yoshikazu Hirai, Ken Ichiro Kamei, Toshiyuki Tsuchiya, Osamu Tabata

Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Trans-epithelial electrical resistance (TEER) is now used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured in Organ-on-a-Chips (OoCs). However, TEER measurements has been limited by considerable errors in data due to improper design of integrating electrodes. Here we report a OoC design method with finite element analysis that achieves the sensitive detection of cell layer and the accurate measurement of TEER values. Our approach has employed an index of sensitivity variation calculated from current density depending on electrodes configuration and fluidic structure, allowing to identify the suitable electrodes design for TEER measurements. To prove proof-of-concept, the impedance spectroscopy measurements were performed in a gut-on-chip. The present approach, supported by the experimental data, will allow a comparison of TEER values across any OoCs as well as real-time assessment of biological functions.

Original language	English (US)
Title of host publication	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	411-414
Number of pages	4
ISBN (Electronic)	9781665419123
DOIs	https://doi.org/10.1109/MEMS51782.2021.9375164
State	Published - Jan 25 2021
Event	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, United States Duration: Jan 25 2021 → Jan 29 2021

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2021-January
ISSN (Print)	1084-6999

Conference

Conference	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
Country/Territory	United States
City	Virtual, Gainesville
Period	1/25/21 → 1/29/21

Keywords

Impedance spectroscopy
Microfluidic device
Organ-on-a-Chip
Trans-epithelial electrical resistance

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMS51782.2021.9375164

Cite this

Miyazaki, T., Yang, J., Imamura, S., Hirai, Y., Kamei, K. I., Tsuchiya, T., & Tabata, O. (2021). Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip. In 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 (pp. 411-414). Article 9375164 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2021-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS51782.2021.9375164

Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip. / Miyazaki, Takashi; Yang, Jiandong; Imamura, Satoshi et al.
34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Institute of Electrical and Electronics Engineers Inc., 2021. p. 411-414 9375164 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2021-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Miyazaki, T, Yang, J, Imamura, S, Hirai, Y, Kamei, KI, Tsuchiya, T & Tabata, O 2021, Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip. in 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021., 9375164, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2021-January, Institute of Electrical and Electronics Engineers Inc., pp. 411-414, 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021, Virtual, Gainesville, United States, 1/25/21. https://doi.org/10.1109/MEMS51782.2021.9375164

Miyazaki T, Yang J, Imamura S, Hirai Y, Kamei KI, Tsuchiya T et al. Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip. In 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Institute of Electrical and Electronics Engineers Inc. 2021. p. 411-414. 9375164. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS51782.2021.9375164

Miyazaki, Takashi ; Yang, Jiandong ; Imamura, Satoshi et al. / Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip. 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Institute of Electrical and Electronics Engineers Inc., 2021. pp. 411-414 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{f9537b5426e84fada743dc1c6b392358,

title = "Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip",

abstract = "Trans-epithelial electrical resistance (TEER) is now used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured in Organ-on-a-Chips (OoCs). However, TEER measurements has been limited by considerable errors in data due to improper design of integrating electrodes. Here we report a OoC design method with finite element analysis that achieves the sensitive detection of cell layer and the accurate measurement of TEER values. Our approach has employed an index of sensitivity variation calculated from current density depending on electrodes configuration and fluidic structure, allowing to identify the suitable electrodes design for TEER measurements. To prove proof-of-concept, the impedance spectroscopy measurements were performed in a gut-on-chip. The present approach, supported by the experimental data, will allow a comparison of TEER values across any OoCs as well as real-time assessment of biological functions.",

keywords = "Impedance spectroscopy, Microfluidic device, Organ-on-a-Chip, Trans-epithelial electrical resistance",

author = "Takashi Miyazaki and Jiandong Yang and Satoshi Imamura and Yoshikazu Hirai and Kamei, {Ken Ichiro} and Toshiyuki Tsuchiya and Osamu Tabata",

note = "Publisher Copyright: {\textcopyright} 2021 IEEE.; 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 ; Conference date: 25-01-2021 Through 29-01-2021",

year = "2021",

month = jan,

day = "25",

doi = "10.1109/MEMS51782.2021.9375164",

language = "English (US)",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "411--414",

booktitle = "34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021",

}

TY - GEN

T1 - Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip

AU - Miyazaki, Takashi

AU - Yang, Jiandong

AU - Imamura, Satoshi

AU - Hirai, Yoshikazu

AU - Kamei, Ken Ichiro

AU - Tsuchiya, Toshiyuki

AU - Tabata, Osamu

PY - 2021/1/25

Y1 - 2021/1/25

N2 - Trans-epithelial electrical resistance (TEER) is now used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured in Organ-on-a-Chips (OoCs). However, TEER measurements has been limited by considerable errors in data due to improper design of integrating electrodes. Here we report a OoC design method with finite element analysis that achieves the sensitive detection of cell layer and the accurate measurement of TEER values. Our approach has employed an index of sensitivity variation calculated from current density depending on electrodes configuration and fluidic structure, allowing to identify the suitable electrodes design for TEER measurements. To prove proof-of-concept, the impedance spectroscopy measurements were performed in a gut-on-chip. The present approach, supported by the experimental data, will allow a comparison of TEER values across any OoCs as well as real-time assessment of biological functions.

AB - Trans-epithelial electrical resistance (TEER) is now used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured in Organ-on-a-Chips (OoCs). However, TEER measurements has been limited by considerable errors in data due to improper design of integrating electrodes. Here we report a OoC design method with finite element analysis that achieves the sensitive detection of cell layer and the accurate measurement of TEER values. Our approach has employed an index of sensitivity variation calculated from current density depending on electrodes configuration and fluidic structure, allowing to identify the suitable electrodes design for TEER measurements. To prove proof-of-concept, the impedance spectroscopy measurements were performed in a gut-on-chip. The present approach, supported by the experimental data, will allow a comparison of TEER values across any OoCs as well as real-time assessment of biological functions.

KW - Impedance spectroscopy

KW - Microfluidic device

KW - Organ-on-a-Chip

KW - Trans-epithelial electrical resistance

UR - http://www.scopus.com/inward/record.url?scp=85103439862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85103439862&partnerID=8YFLogxK

U2 - 10.1109/MEMS51782.2021.9375164

DO - 10.1109/MEMS51782.2021.9375164

M3 - Conference contribution

AN - SCOPUS:85103439862

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 411

EP - 414

BT - 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021

Y2 - 25 January 2021 through 29 January 2021

ER -

Highly Accurate Measurement of Trans-Epithelial Electrical Resistance in Organ-on-a-Chip

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this