Holographic immunoassays: Direct detection of antibodies binding to colloidal spheres

Kaitlynn Snyder, Rushna Quddus, Andrew D. Hollingsworth, Kent Kirshenbaum, David G. Grier

Research output: Contribution to journalArticlepeer-review

Abstract

The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) as they attach to micrometer-diameter colloidal beads coated with protein A. These measurements yield the antibodies' binding rates and can be inverted to obtain the concentration of antibodies in solution. Holographic molecular binding assays therefore can be used to perform fast quantitative immunoassays that are complementary to conventional serological tests.

Original languageEnglish (US)
Pages (from-to)10180-10186
Number of pages7
JournalSoft Matter
Volume16
Issue number44
DOIs
StatePublished - Nov 28 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

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