Holographic Molecular Binding Assays

David G. Grier, Rushna Quddus, Kaitlynn Snyder, Kent Kirshenbaum

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Holographic molecular binding assays detect target molecules binding to the surfaces of specifically functionalized probe beads by measuring the associated increase in bead diameter with holographic video microscopy. Holograms of individual colloidal beads are analyzed by fitting to analytic predictions of the Lorenz-Mie theory of light scattering, yielding measurements of bead diameter with the nanometer precision required to detect binding events. Holographic binding assays share the specificity and robustness of industry-standard bead-based assays. Direct holographic readout eliminates the processing time, expense and uncertainty associated with fluorescent labeling. The underlying technology for holographic particle characterization also has a host of other applications in biopharmaceuticals, semiconductor processing and fundamental research.

Original languageEnglish (US)
Title of host publicationQuantitative Phase Imaging IX
EditorsYang Liu, YongKeun Park
ISBN (Electronic)9781510658837
StatePublished - 2023
EventQuantitative Phase Imaging IX 2023 - San Francisco, United States
Duration: Jan 28 2023Jan 30 2023

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceQuantitative Phase Imaging IX 2023
Country/TerritoryUnited States
CitySan Francisco


  • Holographic microscopy
  • label-free molecular binding assay
  • particle characterization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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