@inproceedings{ebd702df659f469ca70dfb6ff920851b,
title = "Holographic Molecular Binding Assays",
abstract = "Holographic molecular binding assays detect target molecules binding to the surfaces of specifically functionalized probe beads by measuring the associated increase in bead diameter with holographic video microscopy. Holograms of individual colloidal beads are analyzed by fitting to analytic predictions of the Lorenz-Mie theory of light scattering, yielding measurements of bead diameter with the nanometer precision required to detect binding events. Holographic binding assays share the specificity and robustness of industry-standard bead-based assays. Direct holographic readout eliminates the processing time, expense and uncertainty associated with fluorescent labeling. The underlying technology for holographic particle characterization also has a host of other applications in biopharmaceuticals, semiconductor processing and fundamental research.",
keywords = "Holographic microscopy, label-free molecular binding assay, particle characterization",
author = "David Grier and Rushna Quddus and Kaitlynn Snyder and Kent Kirshenbaum",
note = "Funding Information: This work was funded primarily by the National Science Foundation under Awards DMR-2027013 and DMR-2104837 in part by the National Institutes of Health SBIR program of the National Institutes of Health under Award No. R44TR001590. The Spheryx xSight used for this study was purchase by the NYU MRSEC as shared instrumentation under NSF Award No. DMR-1420073. The Titan Xp and Titan RTX GPUs used for this work were provided by GPU Grants from NVIDIA. The custom holographic characterization instrument was constructed with support from the MRI program of the NSF under Award Number DMR-0922680 and is maintained by NYU{\textquoteright}s Center for Soft Matter Research as shared instrumentation. Publisher Copyright: {\textcopyright} 2023 SPIE.; Quantitative Phase Imaging IX 2023 ; Conference date: 28-01-2023 Through 30-01-2023",
year = "2023",
doi = "10.1117/12.2671003",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Yang Liu and YongKeun Park",
booktitle = "Quantitative Phase Imaging IX",
}