Host genes that influence transposition in yeast: The abundance of a rare tRNA regulates Ty1 transposition frequency

Hua Xu; Jef D. Boeke

doi:10.1073/pnas.87.21.8360

Host genes that influence transposition in yeast: The abundance of a rare tRNA regulates Ty1 transposition frequency

Hua Xu, Jef D. Boeke

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single-copy tRNA_CCU^Arg gene as one of the genes that reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNA_CCU^Arg in yeast cells.

Original language	English (US)
Pages (from-to)	8360-8364
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	87
Issue number	21
DOIs	https://doi.org/10.1073/pnas.87.21.8360
State	Published - 1990

Keywords

Retrotransposon
Saccharomyces cerevisiae
Translational frameshifting

ASJC Scopus subject areas

General

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10.1073/pnas.87.21.8360

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N2 - Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single-copy tRNACCUArg gene as one of the genes that reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNACCUArg in yeast cells.

AB - Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single-copy tRNACCUArg gene as one of the genes that reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNACCUArg in yeast cells.

KW - Retrotransposon

KW - Saccharomyces cerevisiae

KW - Translational frameshifting

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Host genes that influence transposition in yeast: The abundance of a rare tRNA regulates Ty1 transposition frequency

Abstract

Keywords

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