Host genes that influence transposition in yeast: The abundance of a rare tRNA regulates Ty1 transposition frequency

Hua Xu, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single-copy tRNACCUArg gene as one of the genes that reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNACCUArg in yeast cells.

    Original languageEnglish (US)
    Pages (from-to)8360-8364
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume87
    Issue number21
    DOIs
    StatePublished - 1990

    Keywords

    • Retrotransposon
    • Saccharomyces cerevisiae
    • Translational frameshifting

    ASJC Scopus subject areas

    • General

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