Hotspot Wizard-informed engineering of a hyperthermophilic β-glucosidase for enhanced enzyme activity at low temperatures

Biochemistry, Genetics and Molecular Biology

• Enzyme 100%
• Enzyme Activity 100%
• Glucosidase 100%
• Thermostability 50%
• Mutagenesis 16%
• Protein Structure 16%
• Wild Type 16%
• Active Site 16%
• Enzyme Specificity 16%
• Pyrococcus furiosus 16%
• Environmental Temperature 16%

Keyphrases

• Hyperthermophilic Enzymes 100%
• Wizard 100%
• Enhanced Enzyme Activity 100%
• Low-temperature Activity 25%
• Mutagenesis Library 25%
• Saturation mutagenesis 25%
• Industrial Enzymes 25%

Immunology and Microbiology

• Enzyme Activity 100%
• Thermostability 100%
• Wild Type 33%
• Mutagenesis 33%
• Pyrococcus furiosus 33%
• Enzyme Specificity 33%
• Protein Structure 33%
• Environmental Temperature 33%