TY - JOUR
T1 - Hundreds of microsatellites for genotyping Plasmodium yoelii parasites
AU - Li, Jian
AU - Zhang, Yanhui
AU - Liu, Shengfa
AU - Hong, Lingxian
AU - Sullivan, Margery
AU - McCutchan, Thomas F.
AU - Carlton, Jane M.
AU - Su, Xin zhuan
N1 - Funding Information:
We thank Professors Weiqing Pan and Fusheng Huang for making the parasite lines available to us, Dr. S. Pattaradilokrat for constructive comments, and NIAID intramural editor Brenda Rae Marshall for assistance. This study was supported by the National Basic Research Program of China, 973 Program 2007CB513103; by a graduate student fund from Xiamen University, China; and by the Intramural Research Program of the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health.
PY - 2009/8
Y1 - 2009/8
N2 - Genetic crosses have been employed to study various traits of rodent malaria parasites and to locate loci that contribute to drug resistance, immune protection, and disease virulence. Compared with human malaria parasites, genetic crossing of rodent malaria parasites is more easily performed; however, genotyping methods using microsatellites (MSs) or large-scale single nucleotide polymorphisms (SNPs) that have been widely used in typing Plasmodium falciparum are not available for rodent malaria species. Here we report a genome-wide search of the Plasmodium yoelii yoelii (P. yoelii) genome for simple sequence repeats (SSRs) and the identification of nearly 600 polymorphic MS markers for typing the genomes of P. yoelii and Plasmodium berghei. The MS markers are randomly distributed across the 14 physical chromosomes assembled from genome sequences of three rodent malaria species, although some variations in the numbers of MS expected according to chromosome size exist. The majority of the MS markers are AT-rich repeats, similar to those found in the P. falciparum genome. The MS markers provide an important resource for genotyping, lay a foundation for developing linkage maps, and will greatly facilitate genetic studies of P. yoelii.
AB - Genetic crosses have been employed to study various traits of rodent malaria parasites and to locate loci that contribute to drug resistance, immune protection, and disease virulence. Compared with human malaria parasites, genetic crossing of rodent malaria parasites is more easily performed; however, genotyping methods using microsatellites (MSs) or large-scale single nucleotide polymorphisms (SNPs) that have been widely used in typing Plasmodium falciparum are not available for rodent malaria species. Here we report a genome-wide search of the Plasmodium yoelii yoelii (P. yoelii) genome for simple sequence repeats (SSRs) and the identification of nearly 600 polymorphic MS markers for typing the genomes of P. yoelii and Plasmodium berghei. The MS markers are randomly distributed across the 14 physical chromosomes assembled from genome sequences of three rodent malaria species, although some variations in the numbers of MS expected according to chromosome size exist. The majority of the MS markers are AT-rich repeats, similar to those found in the P. falciparum genome. The MS markers provide an important resource for genotyping, lay a foundation for developing linkage maps, and will greatly facilitate genetic studies of P. yoelii.
KW - Genetic markers
KW - Genotyping
KW - MS
KW - Rodent malaria parasite
KW - Simple sequence repeat (SSR)
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U2 - 10.1016/j.molbiopara.2009.03.011
DO - 10.1016/j.molbiopara.2009.03.011
M3 - Article
C2 - 19450732
AN - SCOPUS:67349182636
VL - 166
SP - 153
EP - 158
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
SN - 0166-6851
IS - 2
ER -