Hyaluronan and Hyaluronan Fragments

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The glycosaminoglycan hyaluronan (HA) is a key component of the microenvironment surrounding cells. In healthy tissues, HA molecules have extremely high molecular mass and consequently large hydrodynamic volumes. Tethered to the cell surface by clustered receptor proteins, HA molecules crowd each other, as well as other macromolecular species. This leads to severe nonideality in physical properties of the biomatrix, because steric exclusion leads to an increase in effective concentration of the macromolecules. The excluded volume depends on both polymer concentration and hydrodynamic volume/molecular mass. The biomechanical properties of the extracellular matrix, tissue hydration, receptor clustering, and receptor–ligand interactions are strongly affected by the presence of HA and by its molecular mass. In inflammation, reactive oxygen and nitrogen species fragment the HA chains. Depending on the rate of chain degradation relative to the rates of new synthesis and removal of damaged chains, short fragments of the HA molecules can be present at significant levels. Not only are the physical properties of the extracellular matrix affected, but the HA fragments decluster their primary receptors and act as endogenous danger signals. Bioanalytical methods to isolate and quantify HA fragments have been developed to determine profiles of HA content and size in healthy and diseased biological fluids and tissues. These methods have potential use in medical diagnostic tests. Therapeutic agents that modulate signaling by HA fragments show promise in wound healing and tissue repair without fibrosis.

Original languageEnglish (US)
Title of host publicationAdvances in Carbohydrate Chemistry and Biochemistry
PublisherAcademic Press Inc.
Pages1-59
Number of pages59
DOIs
StatePublished - 2017

Publication series

NameAdvances in Carbohydrate Chemistry and Biochemistry
Volume74
ISSN (Print)0065-2318

Keywords

  • Biotherapeutics
  • Cell receptors
  • Cell signaling
  • Extracellular matrix
  • Extracellular vesicles
  • Free radicals
  • Glycosaminoglycan
  • Hyaluronan
  • Inflammation
  • Macromolecular crowding
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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  • Cite this

    Cowman, M. K. (2017). Hyaluronan and Hyaluronan Fragments. In Advances in Carbohydrate Chemistry and Biochemistry (pp. 1-59). (Advances in Carbohydrate Chemistry and Biochemistry; Vol. 74). Academic Press Inc.. https://doi.org/10.1016/bs.accb.2017.10.001