Hyaluronan Isolation from Mouse Mammary Gland

Cornelia Tolg, Mary Cowman, Eva A. Turley

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is proinflammatory, native high molecular weight HA suppresses inflammation. Therefore, it is important to analyze HA size distribution when studying the role of HA in tissue homeostasis and pathology. This protocol describes isolation of HA from mouse mammary glands but can also be applied to other tissues. The quality of the isolated HA is sufficient to analyze size distribution by gel electrophoresis ( Calabro et al., 2000 ).

    Original languageEnglish (US)
    JournalBio-protocol
    Volume8
    Issue number11
    DOIs
    StatePublished - 2018

    Fingerprint

    Dive into the research topics of 'Hyaluronan Isolation from Mouse Mammary Gland'. Together they form a unique fingerprint.

    Cite this