Hyaluronan Isolation from Mouse Mammary Gland

Cornelia  Tolg; Mary Cowman; Eva A. Turley

doi:10.21769/BioProtoc.2865

Hyaluronan Isolation from Mouse Mammary Gland

Cornelia Tolg, Mary Cowman, Eva A. Turley

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is proinflammatory, native high molecular weight HA suppresses inflammation. Therefore, it is important to analyze HA size distribution when studying the role of HA in tissue homeostasis and pathology. This protocol describes isolation of HA from mouse mammary glands but can also be applied to other tissues. The quality of the isolated HA is sufficient to analyze size distribution by gel electrophoresis ( Calabro et al., 2000 ).

Original language	English (US)
Journal	Bio-protocol
Volume	8
Issue number	11
DOIs	https://doi.org/10.21769/BioProtoc.2865
State	Published - 2018

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10.21769/BioProtoc.2865

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title = "Hyaluronan Isolation from Mouse Mammary Gland",

abstract = "The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is proinflammatory, native high molecular weight HA suppresses inflammation. Therefore, it is important to analyze HA size distribution when studying the role of HA in tissue homeostasis and pathology. This protocol describes isolation of HA from mouse mammary glands but can also be applied to other tissues. The quality of the isolated HA is sufficient to analyze size distribution by gel electrophoresis ( Calabro et al., 2000 ). ",

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T1 - Hyaluronan Isolation from Mouse Mammary Gland

AU - Tolg, Cornelia

AU - Cowman, Mary

AU - Turley, Eva A.

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N2 - The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is proinflammatory, native high molecular weight HA suppresses inflammation. Therefore, it is important to analyze HA size distribution when studying the role of HA in tissue homeostasis and pathology. This protocol describes isolation of HA from mouse mammary glands but can also be applied to other tissues. The quality of the isolated HA is sufficient to analyze size distribution by gel electrophoresis ( Calabro et al., 2000 ).

AB - The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is proinflammatory, native high molecular weight HA suppresses inflammation. Therefore, it is important to analyze HA size distribution when studying the role of HA in tissue homeostasis and pathology. This protocol describes isolation of HA from mouse mammary glands but can also be applied to other tissues. The quality of the isolated HA is sufficient to analyze size distribution by gel electrophoresis ( Calabro et al., 2000 ).

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DO - 10.21769/BioProtoc.2865

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SN - 2331-8325

VL - 8

JO - Bio-protocol

JF - Bio-protocol

IS - 11

ER -

Hyaluronan Isolation from Mouse Mammary Gland

Abstract

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