Hydrogen-Deuterium Exchange Analysis of Ligand-Macromolecule Interactions: Ethidium-Deoxyribonucleic Acid System

C. Mandal, S. W. Englander, N. R. Kallenbach

Research output: Contribution to journalArticlepeer-review

Abstract

The interaction between DNA and the intercalating dye, ethidium bromide, was studied by use of a novel approach in which hydrogen-deuterium (H-D) exchange between ethidium amino groups and solvent was measured spectrophotometrically in a stopped-flow mode. The method depends on the fact that ethidium H exchange is greatly slowed on complexation with DNA and that H-D exchange kinetics of the chromophore can be monitored via an accompanying change in its spectral absorbance. The H-D exchange dependent spectral character of ethidium was characterized, and the catalyzed exchange behavior of the free dye and the dye-DNA complex was studied.

Original languageEnglish (US)
Pages (from-to)5819-5825
Number of pages7
JournalBiochemistry
Volume19
Issue number25
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry

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