TY - JOUR
T1 - Hyperspectral autofluorescence imaging of drusen and retinal pigment epithelium in donor eyes with age-related macular degeneration
AU - Tong, Yuehong
AU - Ami, Tal Ben
AU - Hong, Sungmin
AU - Heintzmann, Rainer
AU - Gerig, Guido
AU - Ablonczy, Zsolt
AU - Curcio, Christine A.
AU - Ach, Thomas
AU - Smith, R. Theodore
PY - 2016
Y1 - 2016
N2 - Purpose: To elucidate the molecular pathogenesis of age-related macular degeneration (AMD) and interpretation of fundus autofluorescence imaging, the authors identified spectral autofluorescence characteristics of drusen and retinal pigment epithelium (RPE) in donor eyes with AMD. Methods: Macular RPE/Bruch membrane flat mounts were prepared from 5 donor eyes with AMD. In 12 locations (1-3 per eye), hyperspectral autofluorescence images in 10-nmwavelength steps were acquired at 2 excitation wavelengths (lex 436, 480 nm). A nonnegative tensor factorization algorithm was used to recover 5 abundant emission spectra and their corresponding spatial localizations. Results: At lex 436 nm, the authors consistently localized a novel spectrum (SDr) with a peak emission near 510 nm in drusen and sub-RPE deposits. Abundant emission spectra seen previously (S0 in Bruch membrane and S1, S2, and S3 in RPE lipofuscin/melanolipofuscin, respectively) also appeared in AMD eyes, with the same shapes and peak wavelengths as in normal tissue. Lipofuscin/melanolipofuscin spectra localizations in AMD eyes varied widely in their overlap with drusen, ranging from none to complete. Conclusion: An emission spectrum peaking at-510 nm (lex 436 nm) appears to be sensitive and specific for drusen and sub-RPE deposits. One or more abundant spectra from RPE organelles exhibit characteristic relationships with drusen.
AB - Purpose: To elucidate the molecular pathogenesis of age-related macular degeneration (AMD) and interpretation of fundus autofluorescence imaging, the authors identified spectral autofluorescence characteristics of drusen and retinal pigment epithelium (RPE) in donor eyes with AMD. Methods: Macular RPE/Bruch membrane flat mounts were prepared from 5 donor eyes with AMD. In 12 locations (1-3 per eye), hyperspectral autofluorescence images in 10-nmwavelength steps were acquired at 2 excitation wavelengths (lex 436, 480 nm). A nonnegative tensor factorization algorithm was used to recover 5 abundant emission spectra and their corresponding spatial localizations. Results: At lex 436 nm, the authors consistently localized a novel spectrum (SDr) with a peak emission near 510 nm in drusen and sub-RPE deposits. Abundant emission spectra seen previously (S0 in Bruch membrane and S1, S2, and S3 in RPE lipofuscin/melanolipofuscin, respectively) also appeared in AMD eyes, with the same shapes and peak wavelengths as in normal tissue. Lipofuscin/melanolipofuscin spectra localizations in AMD eyes varied widely in their overlap with drusen, ranging from none to complete. Conclusion: An emission spectrum peaking at-510 nm (lex 436 nm) appears to be sensitive and specific for drusen and sub-RPE deposits. One or more abundant spectra from RPE organelles exhibit characteristic relationships with drusen.
KW - Age-related macular degeneration
KW - Autofluorescence
KW - Bruch membrane
KW - Drusen
KW - Fluorophores
KW - Hyperspectral imaging
KW - Lipofuscin
KW - Nonnegative tensor factorization
KW - Retinal pigment epithelium
KW - Sub-retinal pigment epithelium deposits
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U2 - 10.1097/IAE.0000000000001325
DO - 10.1097/IAE.0000000000001325
M3 - Article
C2 - 28005671
AN - SCOPUS:84991512355
SN - 0275-004X
VL - 36
SP - S127-S136
JO - Retina
JF - Retina
ER -