Identification and developmental expression of Ci-msxb: A novel homologue of Drosophila msh gene in Ciona intestinalis

Francesco Aniello, Annamaria Locascio, Maria Grazia Villani, Anna Di Gregorio, Laura Fucci, Margherita Branno

Research output: Contribution to journalArticlepeer-review

Abstract

We report the cloning and expression pattern of Ci-msxb the second Ciona intestinalis homeobox gene homologue to the Drosophila muscle segment homeobox (msh) gene. Northern blot analysis showed that transcripts appeared at gastrula stage, peaked in the early tailbud and decreased during the tailed stages. Whole mount in situ hybridization showed that the Ci-msxb expression first is detected at 110 cell-stage in the blastomeres that are precursors of different tissue (muscle, spinal cord, endodermal strand, brain, mesenchyme, pigmented cells and primordial pharynx). Transcript level declined in mesoderm cells after the completion of gastrulation, but mRNAs were still present in the folding neural plate during neurulation and in the pigmented cells. Later, at larval stage, transcripts were present around the otolith and ocellus, in a restricted part of the nervous system and in the primordial pharynx; the gene expression was conserved after metamorphosis in the juvenile. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)123-126
Number of pages4
JournalMechanisms of Development
Volume88
Issue number1
DOIs
StatePublished - Oct 1 1999

Keywords

  • Ciona intestinalis
  • Development
  • Homeobox-containing gene
  • Msx
  • Nervous system
  • Neural gland
  • Ocellus
  • Otolith
  • Pharynx
  • Urochordata

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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