TY - JOUR
T1 - Identification of a Stat gene that functions in Drosophila development
AU - Yan, Riqiang
AU - Small, Stephen
AU - Desplan, Claude
AU - Dearolf, Charles R.
AU - Darnell, James E.
N1 - Funding Information:
We are greatly indebted to Nathalie Dostatni and Anne Goriely for assistance in chromosomal localization of D-Stat, in situ staining, and examining and photographing stained embryos; Monica Boyle for assistance in double staining; Guojun Sheng for assistance in cell culture; Ed Lewis and Sue Celniker for assistance with the analysis of stat HJ salivary gland chromosomes; and Ruth Lehmann for analysis of the stat HJ cuticle preparations. We thank Terry Turner for excellent technical assistance. We greatly appreciate numerous fruitful discussions with Steve DiNardo, Nathalie Dostatni, Anne Goriely, Guojun Sheng, Susie Jun, David Wilson, Claudio Bertuccioli, and Curt Horvath and thank Michael Levine for sharing unpublished sequence information. We thank Simon Kidd (Rockefeller University) for the Drosophila embryonic cDNA library, Dr. Norbert Perrimon (Harvard Medical School) for hop cDNA, Dr. James Posakany (University of California, San Diego) for Hairless cDNA, and Dr. Toby Lieber (Rockefeller University) for RP49 cDNA. This work was supported by National Institutes of Health grants AI32489 and AI34420 to J. E. D., GM1946 to S. S., and HL48823 to C. R. D.; C. R. D. also received support from the Harvard Joint Center for Radiation Therapy. C. D. is supported by the Howard Hughes Medical Institute. R. Y. is an Aaron Diamond Foundation postdoctoral fellow.
PY - 1996/2/9
Y1 - 1996/2/9
N2 - A Drosophila Stat gene (D-Stat) with a zygotic segmental expression pattern was identified. This protein becomes phosphorylated on Tyr-704 when coexpressed in Schneider cells with a Drosophila Janus kinase (JAK), Hopscotch (HOP). The phosphorylated protein binds specifically to the consensus sequence TTCCCGGAA. Suppressor mutations of hop(Tum-1), a dominant hyperactive allele of hop whose phenotype is hematocyte overproduction and tumor formation, were selected. One of these mutants, stat(HJ), mapped to the same chromosomal region (92E) as does D-Stat, had an incompletely penetrant pair rule phenotype, and exhibited aberrant expression of the pair rule gene even skipped (eve) at the cellular blastoderm stage. Two D-STAT-binding sites were identified within the eve stripe 3 enhancer region. Mutations in either of the STAT-binding sites greatly decreased the stripe 3 expression in transgenic flies. Clearly, the JAK-STAT pathway is connected to Drosophila early development.
AB - A Drosophila Stat gene (D-Stat) with a zygotic segmental expression pattern was identified. This protein becomes phosphorylated on Tyr-704 when coexpressed in Schneider cells with a Drosophila Janus kinase (JAK), Hopscotch (HOP). The phosphorylated protein binds specifically to the consensus sequence TTCCCGGAA. Suppressor mutations of hop(Tum-1), a dominant hyperactive allele of hop whose phenotype is hematocyte overproduction and tumor formation, were selected. One of these mutants, stat(HJ), mapped to the same chromosomal region (92E) as does D-Stat, had an incompletely penetrant pair rule phenotype, and exhibited aberrant expression of the pair rule gene even skipped (eve) at the cellular blastoderm stage. Two D-STAT-binding sites were identified within the eve stripe 3 enhancer region. Mutations in either of the STAT-binding sites greatly decreased the stripe 3 expression in transgenic flies. Clearly, the JAK-STAT pathway is connected to Drosophila early development.
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U2 - 10.1016/S0092-8674(00)81287-8
DO - 10.1016/S0092-8674(00)81287-8
M3 - Article
C2 - 8608596
AN - SCOPUS:0001514956
SN - 0092-8674
VL - 84
SP - 421
EP - 430
JO - Cell
JF - Cell
IS - 3
ER -