Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis

Dipak Kumar Sahoo, Chinmay Hegde, Madan K. Bhattacharyya

    Research output: Contribution to journalArticlepeer-review


    Introduction: Cold stress adversely affects the growth and development of plants and limits the geographical distribution of many plant species. Accumulation of spontaneous mutations shapes the adaptation of plant species to diverse climatic conditions. Methods: The genome-wide association study of the phenotypic variation gathered by a newly designed phenomic platform with the over six millions single nucleotide polymorphic (SNP) loci distributed across the genomes of 417 Arabidopsis natural variants collected from various geographical regions revealed 33 candidate cold responsive genes. Results: Investigation of at least two independent insertion mutants for 29 genes identified 16 chilling tolerance genes governing diverse genetic mechanisms. Five of these genes encode novel leucine-rich repeat domain-containing proteins including three nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins. Among the 16 identified chilling tolerance genes, ADS2 and ACD6 are the only two chilling tolerance genes identified earlier. Discussion: The 12.5% overlap between the genes identified in this genome-wide association study (GWAS) of natural variants with those discovered previously through forward and reverse genetic approaches suggests that chilling tolerance is a complex physiological process governed by a large number of genetic mechanisms.

    Original languageEnglish (US)
    Article number1094462
    JournalFrontiers in Plant Science
    StatePublished - Jan 12 2023


    • Arabidopsis
    • GWAS
    • NBS-LRR
    • abiotic stress
    • cold tolerance mechanisms
    • mutatnt analyses
    • phenomics platform

    ASJC Scopus subject areas

    • Plant Science


    Dive into the research topics of 'Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis'. Together they form a unique fingerprint.

    Cite this