Identification of neuropeptide-degrading enzymes in the pancreas

H. Terashima, A. Okamoto, D. Menozzi, E. J. Goetzl, N. W. Bunnett

Research output: Contribution to journalArticle

Abstract

Neutral endopeptidase (NEP) and aminopeptidase M (APM) were identified in the pancreas by enzymatic assays and Western blotting. The NEP activity, assessed by the phosphoramidon- and dl-thiorphan-inhibitable degradation of glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamine, was 28.8 pmol/h/μg of pancreatic membrane protein and 124 pmol/h/106 pancreatic acinar cells. The APM enzymatic activity, assessed by the actinonin- and amastatin-inhibitable degradation of Ala-4-methoxy-2-naphthylamine, was 633 pmol/h/μg pancreatic membrane protein and 17.4 nmol/h/106 pancreatic acinar cells. Proteins corresponding to NEP (95 kDa) and APM (140 kDa) were identified in membranes by Western blotting. Both NEP and APM on acinar cells may degrade neuropeptides and regulate their effects on exocrine secretion.

Original languageEnglish (US)
Pages (from-to)741-748
Number of pages8
JournalPeptides
Volume13
Issue number4
DOIs
StatePublished - 1992

Keywords

  • Aminopeptidase M
  • Enkephalins
  • Neutral endopeptidase
  • Pancreatic acinar cells
  • Pancreatic secretion

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Identification of neuropeptide-degrading enzymes in the pancreas'. Together they form a unique fingerprint.

  • Cite this

    Terashima, H., Okamoto, A., Menozzi, D., Goetzl, E. J., & Bunnett, N. W. (1992). Identification of neuropeptide-degrading enzymes in the pancreas. Peptides, 13(4), 741-748. https://doi.org/10.1016/0196-9781(92)90181-2