Identifying individual DNA species in a complex mixture by precisely measuring the spacing between nicking restriction enzymes with atomic force microscope

Jason Reed, Carlin Hsueh, Miu Ling Lam, Rachel Kjolby, Andrew Sundstrom, Bud Mishra, J. K. Gimzewski

Research output: Contribution to journalArticlepeer-review

Abstract

We discuss a novel atomic force microscope-based method for identifying individual short DNA molecules (< 5000 bp) within a complex mixture by measuring the intra-molecular spacing of a few sequence-specific topographical labels in each molecule. Using this method, we accurately determined the relative abundance of individual DNA species in a 15-species mixture, with fewer than 100 copies per species sampled. To assess the scalability of our approach, we conducted a computer simulation, with realistic parameters, of the hypothetical problem of detecting abundance changes in individual gene transcripts between two single-cell human messenger RNA samples, each containing roughly 9000 species. We found that this approach can distinguish transcript species abundance changes accurately in most cases, including transcript isoforms which would be challenging to quantitate with traditional methods. Given its sensitivity and procedural simplicity, our approach could be used to identify transcript-derived complementary DNAs, where it would have substantial technical and practical advantages versus established techniques in situations where sample material is scarce.

Original languageEnglish (US)
Pages (from-to)2341-2350
Number of pages10
JournalJournal of the Royal Society Interface
Volume9
Issue number74
DOIs
StatePublished - Sep 7 2012

Keywords

  • Atomic force microscopy
  • Bioinformatics
  • Bionanoscience

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

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