IL-2 immunotherapy to recently HIV-1 infected adults maintains the numbers of IL-17 expressing CD4+ T (TH17) cells in the periphery

Lishomwa C. Ndhlovu, Elizabeth Sinclair, Lorrie Epling, Qi Xuan Tan, Terence Ho, Aashish R. Jha, Ijeoma Eccles-James, Camilla Tincati, Jay A. Levy, Douglas F. Nixon, Frederick M. Hecht, Jason D. Barbour

Research output: Contribution to journalArticle

Abstract

Little is known about the manipulation of IL-17 producing CD4+ T cells (TH17) on a per-cell basis in humans in vivo. Previous studies on the effects of IL-2 on IL-17 secretion in non-HIV models have shown divergent results. We hypothesized that IL-2 would mediate changes in IL-17 levels among recently HIV-1-infected adults receiving anti-retroviral therapy. We measured cytokine T cell responses to CD3/CD28, HIV-1 Gag, and CMV pp65 stimulation, and changes in multiple CD4+ T cell subsets. Those who received IL-2 showed a robust expansion of naive and total CD4+ T cell counts and T-reg counts. However, after IL-2 treatment, the frequency of TH17 cells declined, while counts of TH17 cells did not change due to an expansion of the CD4+ naïve T cell population (CD27+CD45RA+). Counts of HIV-1 Gag-specific T cells declined modestly, but CMV pp65 and CD3/CD28 stimulated populations did not change. Hence, in contrast with recent studies, our results suggest IL-2 is not a potent in vivo regulator of TH17 cell populations in HIV-1 disease. However, IL-2-mediated T-reg expansions may selectively reduce responses to certain antigen-specific populations, such as HIV-1 Gag.

Original languageEnglish (US)
Pages (from-to)681-692
Number of pages12
JournalJournal of Clinical Immunology
Volume30
Issue number5
DOIs
StatePublished - Sep 2010

Keywords

  • HIV-1
  • Human
  • T cells
  • T-regs
  • anti-retroviral therapy
  • cytokines
  • interleukin-17
  • interleukin-2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'IL-2 immunotherapy to recently HIV-1 infected adults maintains the numbers of IL-17 expressing CD4+ T (T<sub>H</sub>17) cells in the periphery'. Together they form a unique fingerprint.

  • Cite this

    Ndhlovu, L. C., Sinclair, E., Epling, L., Tan, Q. X., Ho, T., Jha, A. R., Eccles-James, I., Tincati, C., Levy, J. A., Nixon, D. F., Hecht, F. M., & Barbour, J. D. (2010). IL-2 immunotherapy to recently HIV-1 infected adults maintains the numbers of IL-17 expressing CD4+ T (TH17) cells in the periphery. Journal of Clinical Immunology, 30(5), 681-692. https://doi.org/10.1007/s10875-010-9432-3