Impaired pain sensation in mice lacking prokineticin 2

Wang Ping Hu, Chengkang Zhang, Jia Da Li, Z. David Luo, Silvia Amadesi, Nigel Bunnett, Qun Yong Zhou

Research output: Contribution to journalArticle

Abstract

Prokineticins (PKs), consisting of PK1 and PK2, are a pair of newly identified regulatory peptides. Two closely related G-protein coupled receptors, PKR1 and PKR2, mediate the signaling of PKs. PKs/PKRs participate in the regulation of diverse biological processes, ranging from development to adult physiology. A number of studies have indicated the involvement of PKs/PKRs in nociception. Here we show that PK2 is a sensitizer for nociception. Intraplantar injection of recombinant PK2 resulted in a strong and localized hyperalgesia with reduced thresholds to nociceptive stimuli. PK2 mobilizes calcium in dissociated dorsal root ganglion (DRG) neurons. Mice lacking the PK2 gene displayed strong reduction in nociception induced by thermal and chemical stimuli, including capsaicin. However, PK2 mutant mice showed no difference in inflammatory response to capsaicin. As the majority of PK2-responsive DRG neurons also expressed transient receptor potential vanilloid (TRPVI) and exhibited sensitivity to capsaicin, TRPVI is likely a significant downstream molecule of PK2 signaling. Taken together, these results reveal that PK2 sensitize nociception without affecting inflammation.

Original languageEnglish (US)
Article number35
JournalMolecular Pain
Volume2
DOIs
StatePublished - Nov 15 2006

ASJC Scopus subject areas

  • Molecular Medicine
  • Cellular and Molecular Neuroscience
  • Anesthesiology and Pain Medicine

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  • Cite this

    Hu, W. P., Zhang, C., Li, J. D., Luo, Z. D., Amadesi, S., Bunnett, N., & Zhou, Q. Y. (2006). Impaired pain sensation in mice lacking prokineticin 2. Molecular Pain, 2, [35]. https://doi.org/10.1186/1744-8069-2-35