TY - JOUR
T1 - Improving spectral resolution in biological solid-state NMR using phase-alternated rCW heteronuclear decoupling
AU - Equbal, Asif
AU - Bjerring, Morten
AU - Madhu, P. K.
AU - Nielsen, Niels Chr
N1 - Funding Information:
The project was supported by grants from the Danish National Research Foundation ( DNRF59 ) and the European Commission under the Seventh Framework Programme (FP7), contract Bio-NMR 261863. We thank Dr. Zdenek Tosner for assistance with the SIMSPON implementation.
Publisher Copyright:
© 2015 Published by Elsevier B.V.
PY - 2015/7/27
Y1 - 2015/7/27
N2 - The successful application of solid-state NMR spectroscopy for structural study of biological macromolecules requires high spectral resolution. In presence of abundant H1 spins, the resolution of the prevailing C13 or N15 chemical shift encoding experiments critically depends on the availability of efficient and robust heteronuclear decoupling methods in addition to the use of high-field instrumentation and fast sample spinning. Robustness of the decoupling method towards alterations in amplitude/offset of radio frequency fields due to varying sample states is important to ensure recording of spectra with high resolution over long sampling periods for insensitive samples. Here, we present a phase-alternated refocused continuous-wave decoupling method offering better resolution, easier setup, and higher robustness than previous methods. Improved decoupling is in part ascribed to more efficient cancellation of the residual heteronuclear, H1-C13, dipolar coupling interactions which are induced by homonuclear, H1-H1, dipolar coupling interactions.
AB - The successful application of solid-state NMR spectroscopy for structural study of biological macromolecules requires high spectral resolution. In presence of abundant H1 spins, the resolution of the prevailing C13 or N15 chemical shift encoding experiments critically depends on the availability of efficient and robust heteronuclear decoupling methods in addition to the use of high-field instrumentation and fast sample spinning. Robustness of the decoupling method towards alterations in amplitude/offset of radio frequency fields due to varying sample states is important to ensure recording of spectra with high resolution over long sampling periods for insensitive samples. Here, we present a phase-alternated refocused continuous-wave decoupling method offering better resolution, easier setup, and higher robustness than previous methods. Improved decoupling is in part ascribed to more efficient cancellation of the residual heteronuclear, H1-C13, dipolar coupling interactions which are induced by homonuclear, H1-H1, dipolar coupling interactions.
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U2 - 10.1016/j.cplett.2015.07.008
DO - 10.1016/j.cplett.2015.07.008
M3 - Article
AN - SCOPUS:84937784542
SN - 0009-2614
VL - 635
SP - 339
EP - 344
JO - Chemical Physics Letters
JF - Chemical Physics Letters
ER -