Abstract
A plasmid bearing a GAL1::Tyl fusion that is competent to transpose was mutagenized by insertion of oligodeoxyribo-nucleotides that precisely introduce four or five codons semirandomly throughout the plasmid. Approximately one quarter of these resulted in inactivation of transposition; these include inactivating insertions in both the TYA and TYB genes, corresponding to retroviral gag and pol genes. Examples of transposition-inactivating mutations map within each of the known or proposed functional domains of TYB, suggesting that these are all required for retrotransposition. All of the transposition-inactivating mutations were found to be recessive with the exception of a single mutation in TYA. The remaining mutations have slightly deleterious to no effect on Ty1 transposition.
Original language | English (US) |
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Pages (from-to) | 9-18 |
Number of pages | 10 |
Journal | Gene |
Volume | 139 |
Issue number | 1 |
DOIs | |
State | Published - Feb 11 1994 |
Keywords
- Retrotransposons
- codon insertion mutations
- vSaccharomyces cerevisiae
ASJC Scopus subject areas
- Genetics