In-frame linker insertion mutagenesis of yeast transposon Ty1: mutations, transposition and dominance

G. M. Monokian, L. T. Braiterman, J. D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A plasmid bearing a GAL1::Tyl fusion that is competent to transpose was mutagenized by insertion of oligodeoxyribo-nucleotides that precisely introduce four or five codons semirandomly throughout the plasmid. Approximately one quarter of these resulted in inactivation of transposition; these include inactivating insertions in both the TYA and TYB genes, corresponding to retroviral gag and pol genes. Examples of transposition-inactivating mutations map within each of the known or proposed functional domains of TYB, suggesting that these are all required for retrotransposition. All of the transposition-inactivating mutations were found to be recessive with the exception of a single mutation in TYA. The remaining mutations have slightly deleterious to no effect on Ty1 transposition.

    Original languageEnglish (US)
    Pages (from-to)9-18
    Number of pages10
    JournalGene
    Volume139
    Issue number1
    DOIs
    StatePublished - Feb 11 1994

    Keywords

    • Retrotransposons
    • codon insertion mutations
    • vSaccharomyces cerevisiae

    ASJC Scopus subject areas

    • Genetics

    Fingerprint Dive into the research topics of 'In-frame linker insertion mutagenesis of yeast transposon Ty1: mutations, transposition and dominance'. Together they form a unique fingerprint.

    Cite this