In Situ Detection of Lytic Epstein-Barr Virus Infection: Expression of the NotI Early Gene and Viral Interleukin-IO Late Gene in Clinical Specimens

Judith J. Ryon, S. Diane Hayward, Eithne M E MacMahon, Risa B. Mann, Yun Ling, Patricia Charache, Joan Andersen Phelan, George Miller, Richard F. Ambinder

Research output: Contribution to journalArticlepeer-review

Abstract

Riboprobes that detect two genes expressed only during productive infection were developed to characterize the clinical spectrum of Epstein-Barr virus (EBV) lytic infection and identify diseases that may be responsive to antiviral drug therapy. The NotI antisense probe hybridizes to tandem repeats in the abundant early lytic cycle BHLF1 mRNA. Transcripts were detected in lytically infected cell lines, AIDS-associated oral hairy leukoplakia, bone marrow of a patient with virus-associated hemophagocytic syndrome, and spleen of an AIDS patient but not in EBVpositive primary central nervous system lymphomas or in circulating EBV-infected B cells from a patient with acute infectious mononucleosis. The viral (v) interleukin-10 (IL-10) probe hybridizes to the unique 5′ end of the late lytic cycle BCRFI mRNA, which encodes a protein homologous to the human cytokine IL-10. The vIL-10 probe detected transcripts in lytically infected cell lines and within the differentiated layers of oral hairy leukoplakia.

Original languageEnglish (US)
Pages (from-to)345-351
Number of pages7
JournalJournal of Infectious Diseases
Volume168
Issue number2
DOIs
StatePublished - Aug 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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