In vitro evolution suggests multiple origins for the hammerhead ribozyme

K. Salehi-Ashtiani, J. W. Szostak

Research output: Contribution to journalArticlepeer-review


The hammerhead ribozyme was originally discovered in a group of RNAs associated with plant viruses, and has subsequently been identified in the genome of the newt (Notophthalamus viridescens), in schistosomes and in cave crickets (Dolichopoda species). The sporadic occurrence of this self-cleaving RNA motif in highly divergent organisms could be a consequence of the very early evolution of the hammerhead ribozyme, with all extant examples being descended from a single ancestral progenitor. Alternatively, the hammerhead ribozyme may have evolved independently many times. To better understand the observed distribution of hammerhead ribozymes, we used in vitro selection to search an unbiased sample of random sequences for comparably active self-cleaving motifs. Here we show that, under near-physiological conditions, the hammerhead ribozyme motif is the most common (and thus the simplest) RNA structure capable of self-cleavage at biologically observed rates. Our results suggest that the evolutionary process may have been channelled, in nature as in the laboratory, towards repeated selection of the simplest solution to a biochemical problem.

Original languageEnglish (US)
Pages (from-to)82-84
Number of pages3
Issue number6859
StatePublished - Nov 1 2001

ASJC Scopus subject areas

  • General


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